( We provide with REG3D qPCR primers for gene expression analysis, MP200532 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|小鼠 REG3D 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||MG50540-ACG|
|小鼠 REG3D 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||MG50540-ACR|
|小鼠 REG3D 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||MG50540-CF|
|小鼠 REG3D 基因ORF全长cDNA克隆(表达载体), C-His 标签||MG50540-CH|
|小鼠 REG3D 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||MG50540-CM|
|小鼠 REG3D 基因ORF全长cDNA克隆(表达载体), C-HA 标签||MG50540-CY|
|小鼠 REG3D 基因ORF全长cDNA(克隆载体)||MG50540-M|
|小鼠 REG3D 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||MG50540-NF|
|小鼠 REG3D 基因ORF全长cDNA克隆(表达载体), N-His 标签||MG50540-NH|
|小鼠 REG3D 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||MG50540-NM|
|小鼠 REG3D 基因ORF全长cDNA克隆(表达载体), N-HA 标签||MG50540-NY|
|小鼠 REG3D 基因ORF全长cDNA克隆(表达载体)||MG50540-UT|
Regenerating islet-derived 3 delta (REG3D) is a member of the secreted Reg superfamily and contains one typical C-type lectin domain. Regenerating gene (Reg), first isolated from a regenerating islet cDNA library, encodes a secretory protein with a growth stimulating effect on pancreatic beta cells. Reg and Reg-related genes which were expressed in various organs have been revealed to constitute a multigene family, the Reg family, which consists of four subtypes (types I, II, III, IV) based on the primary structures of the encoded proteins of the genes, which are associated with tissue repair and have been directly implicated in pancreatic beta-cell regeneration. Reg proteins are expressed in various organs and are involved in cancers and neurodegenerative diseases. They display a typical C-type lectin-like domain but possess additional highly conserved amino acids.