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小鼠 Semaphorin 4A/SEMA4A/Semaphorin B transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-Myc 标签

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Mouse SEMA4A cDNA Clone产品信息
NCBI注册码:NM_013658.3
参考序列ORF长度:2283bp
cDNA基因描述:Full length Clone DNA of Mus musculus sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A, transcript variant 1 with C terminal Myc tag.
分子别称:SemB, Semab, AI132332, Sema4a
分子种属:Mouse
载体:pCMV3-C-Myc
质粒:
限制性酶切位点:
Tag序列:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
序列信息:
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

小鼠 Semaphorin 4A/SEMA4A/Semaphorin B transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-Myc 标签 on other vectors
小鼠 Semaphorin 4A/SEMA4A/Semaphorin B transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签MG50330-ACG2070
小鼠 Semaphorin 4A/SEMA4A/Semaphorin B transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签MG50330-ACR2070
小鼠 Semaphorin 4A/SEMA4A/Semaphorin B transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签MG50330-CF1890
小鼠 Semaphorin 4A/SEMA4A/Semaphorin B transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-His 标签MG50330-CH1890
小鼠 Semaphorin 4A/SEMA4A/Semaphorin B transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-Myc 标签MG50330-CM1890
小鼠 Semaphorin 4A/SEMA4A/Semaphorin B transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-HA 标签MG50330-CY1890
小鼠 Semaphorin 4A/SEMA4A/Semaphorin B transcript variant 1 基因ORF全长cDNA(克隆载体)MG50330-M920
小鼠 Semaphorin 4A/SEMA4A/Semaphorin B transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-Flag 标签MG50330-NF1890
小鼠 Semaphorin 4A/SEMA4A/Semaphorin B transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-His 标签MG50330-NH1890
小鼠 Semaphorin 4A/SEMA4A/Semaphorin B transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-Myc 标签MG50330-NM1890
小鼠 Semaphorin 4A/SEMA4A/Semaphorin B transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-HA 标签MG50330-NY1890
小鼠 Semaphorin 4A/SEMA4A/Semaphorin B transcript variant 1 基因ORF全长cDNA克隆(表达载体)MG50330-UT1890
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研究背景

Semaphorin-4A, also known as Semaphorin-B, SEMA4A, Sema B and SEMAB, is a single-pass type I  membrane protein which belongs to the semaphorin family. It inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. Semaphorin-4A / SEMA4A contains one Ig-like C2-type (immunoglobulin-like) domain, one PSI domain and one Sema domain. Defects in SEMA4A are the cause of retinitis pigmentosa type 35 (RP35) which leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. Defects in SEMA4A are also the cause of cone-rod dystrophy type 10 (CORD10) which are inherited retinal dystrophies belonging to the group of pigmentary retinopathies. CORDs are characterized by retinal pigment deposits visible on fundus examination, predominantly in the macular region, and initial loss of cone photoreceptors followed by rod degeneration.

Semaphorins are secreted, transmembrane, and GPI-linked proteins, defined by cysteine-rich semaphorin protein domains, that have important roles in a variety of tissues. Humans have 20 semaphorins, Drosophila has five, and two are known from DNA viruses. Semaphorins are found in nematodes and crustaceans but not in non-animals. They are grouped into eight classes on the basis of phylogenetic tree analyses and the presence of additional protein motifs. Semaphorins have been implicated in diverse developmental processes such as axon guidance during nervous system development and regulation of cell migration.

参考资料
  • Clark H.F., et al., 2003, Genome Res. 13: 2265-2270.
  • Ota T., et al., 2004,Nat. Genet. 36: 40-45.
  • Neufeld, G. et al., 2005, Front Biosci. 10 : 751-60.
  • Fiore,R. et al., 2005, Mol Cell Biol. 25 (6):2310-9.
  • Abid A., et al., 2006, J. Med. Genet. 43:378-381.
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