( We provide with SLAMF7 qPCR primers for gene expression analysis, MP200230 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|小鼠 CRACC/SLAM7/CD319 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||MG50201-ACG|
|小鼠 CRACC/SLAM7/CD319 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||MG50201-ACR|
|小鼠 CRACC/SLAM7/CD319 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||MG50201-CF|
|小鼠 CRACC/SLAM7/CD319 基因ORF全长cDNA克隆(表达载体), C-His 标签||MG50201-CH|
|小鼠 CRACC/SLAM7/CD319 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||MG50201-CM|
|小鼠 CRACC/SLAM7/CD319 基因ORF全长cDNA克隆(表达载体), C-HA 标签||MG50201-CY|
|小鼠 CRACC/SLAM7/CD319 基因ORF全长cDNA(克隆载体)||MG50201-M|
|小鼠 CRACC/SLAM7/CD319 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||MG50201-NF|
|小鼠 CRACC/SLAM7/CD319 基因ORF全长cDNA克隆(表达载体), N-His 标签||MG50201-NH|
|小鼠 CRACC/SLAM7/CD319 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||MG50201-NM|
|小鼠 CRACC/SLAM7/CD319 基因ORF全长cDNA克隆(表达载体), N-HA 标签||MG50201-NY|
|小鼠 CRACC/SLAM7/CD319 基因ORF全长cDNA克隆(表达载体)||MG50201-UT|
SLAM family member 7 (SLAMF7), also known as CRACC, CD319, CD2-like receptor-activating cytotoxic cells, and CS1, is a single-pass type I membrane protein and a member of the CD2 family of cell surface receptors. SLAMF7 is expressed in NK cells, activated B-cells, NK-cell line but not in promyelocytic, B-cell lines, or T-cell lines. Although the cytoplasmic domain of CS1 contains immunoreceptor tyrosine-based switch motifs (ITSM), which enables to recruite signaling lymphocyte activation molecule (SLAM)-associated protein (SAP/SH2D1A), it activates NK cells in the absence of a functional SAP. CS1 is a self ligand and homophilic interaction of CS1 regulates NK cell cytolytic activity. CRACC positively regulated natural killer cell functions by a mechanism dependent on the adaptor EAT-2 but not the related adaptor SAP. However, in the absence of EAT-2, CRACC potently inhibited natural killer cell function. It was also inhibitory in T cells, which are typically devoid of EAT-2. Thus, CRACC can exert activating or inhibitory influences on cells of the immune system depending on cellular context and the availability of effector proteins.