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小鼠 SRC/Proto-oncogene c-Src transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-HA 标签

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Mouse SRC cDNA Clone产品信息
NCBI注册码:NM_009271.3
参考序列ORF长度:1626bp
cDNA基因描述:Full length Clone DNA of Mus musculus Rous sarcoma oncogene transcript variant 1 with C terminal HA tag.
分子别称:AW259666, pp60c-src, Src
分子种属:Mouse
载体:pCMV3-C-HA
质粒:
限制性酶切位点:
Tag序列:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
序列信息:
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

小鼠 SRC/Proto-oncogene c-Src transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-HA 标签 on other vectors
小鼠 SRC/Proto-oncogene c-Src transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签MG51118-ACG2070
小鼠 SRC/Proto-oncogene c-Src transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签MG51118-ACR2070
小鼠 SRC/Proto-oncogene c-Src transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签MG51118-ANG2070
小鼠 SRC/Proto-oncogene c-Src transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签MG51118-ANR2070
小鼠 SRC/Proto-oncogene c-Src transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签MG51118-CF1890
小鼠 SRC/Proto-oncogene c-Src transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-His 标签MG51118-CH1890
小鼠 SRC/Proto-oncogene c-Src transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-Myc 标签MG51118-CM1890
小鼠 SRC/Proto-oncogene c-Src transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-HA 标签MG51118-CY1890
小鼠 SRC/Proto-oncogene c-Src transcript variant 1 基因ORF全长cDNA(克隆载体)MG51118-G920
小鼠 SRC/Proto-oncogene c-Src transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签MG51118-G-F1890
小鼠 SRC/Proto-oncogene c-Src transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-Flag 标签MG51118-NF1890
小鼠 SRC/Proto-oncogene c-Src transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-His 标签MG51118-NH1890
小鼠 SRC/Proto-oncogene c-Src transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-Myc 标签MG51118-NM1890
小鼠 SRC/Proto-oncogene c-Src transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-HA 标签MG51118-NY1890
小鼠 SRC/Proto-oncogene c-Src transcript variant 1 基因ORF全长cDNA克隆(表达载体)MG51118-UT1890
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研究背景

Proto-oncogene tyrosine-protein kinase SRC is a hydrophobic protein belonging to the SRC family kinase including nine members that is a family of non-receptor tyrosine kinases. SRC protein may exist in different forms: C-SRC and V-SRC. C-SRC is only activated under certain circumstances where it is required such as growth factor signaling, while V-SRC is a constitutively active as opposed to normal SRC (C-SRC). Thus, V-SRC is an instructive example of an oncogene protein kinase whereas C-SRC is a proto-oncogene protein kinase. Inhibition of SRC with NR2A tyrosine phosphorylation mediated by PSD-95 may contribute to the lithium-induced downregulation of NMDA receptor function and provide neuroprotection against excitotoxicity.

参考资料
  • Juan Ma. et al., 2003, Neuroscience Letters. 348 (3): 185-189.
  • Czernilofsky AP. et al., 1980, Nature. 287: 198-203.
  • Beischlag TV. et al., 2002, Molecular and cellular biology. 22 (12): 4319-33.
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    货号: MG51118-CY
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