|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|小鼠 SerpinB8 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||MG50215-ACG|
|小鼠 SerpinB8 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||MG50215-ACR|
|小鼠 SerpinB8 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签||MG50215-ANG|
|小鼠 SerpinB8 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签||MG50215-ANR|
|小鼠 SerpinB8 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||MG50215-CF|
|小鼠 SerpinB8 基因ORF全长cDNA克隆(表达载体), C-His 标签||MG50215-CH|
|小鼠 SerpinB8 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||MG50215-CM|
|小鼠 SerpinB8 基因ORF全长cDNA克隆(表达载体), C-HA 标签||MG50215-CY|
|小鼠 SerpinB8 基因ORF全长cDNA(克隆载体)||MG50215-M|
|小鼠 SerpinB8 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||MG50215-NF|
|小鼠 SerpinB8 基因ORF全长cDNA克隆(表达载体), N-His 标签||MG50215-NH|
|小鼠 SerpinB8 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||MG50215-NM|
|小鼠 SerpinB8 基因ORF全长cDNA克隆(表达载体), N-HA 标签||MG50215-NY|
|小鼠 SerpinB8 基因ORF全长cDNA克隆(表达载体)||MG50215-UT|
Serpins are the largest and most diverse family of serine protease inhibitors which are involved in a number of fundamental biological processes such as blood coagulation, complement activation, fibrinolysis, angiogenesis, inflammation and tumor suppression and are expressed in a cell-specific manner.
Mouse SerpinB8, also known as Cytoplasmic antiproteinase 2, Peptidase inhibitor 8, SerpinB8, PI-8, SERPINB8 and CAP2, is a member of the Serpin superfamily. SERPINB8 was broadly expressed. In normal neuroendocrine tissues, strongest SerpinB8 expression was detected in islets of Langerhans of the pancreas. Moderate SerpinB8 expression was observed in neuroendocrine cells of the thyroid, adrenal cortex, colon, and pituitary gland. In the pancreas, SerpinB8 is specifically expressed by insulin-producing beta cells, and can be used as an additional diagnostic immunohistochemical marker. Mouse SerpinB8 distribution alters during kidney regeneration, possibly to control a prohormone convertase involved in inflammation or tissue repair.