Smad2 cDNA ORF Clone, Mouse, untagged

Cat: MG50727-UT
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Smad2 cDNA ORF Clone, Mouse, untagged 基本信息
基因
种属
Mouse
NCBI 参考序列号
参考序列ORF长度
1404 bp
序列描述
Identical with the Gene Bank Ref. ID sequence except for the point mutations: 219A/G,573G/T,888G/A均 not causing the amino acid variation.
描述
Full length Clone DNA of Mouse Mothers against decapentaplegic homolog 2.
质粒
启动子
Enhanced CMV promoter
载体
pCMV3-untagged
限制性酶切位点
KpnI + XbaI(6.1kb+1.4kb)
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
抗生素(大肠杆菌)
Ampicillin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.
Smad2 cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

Smad2 cDNA ORF Clone, Mouse, untagged Validated Images
other Green fluorescent protein / GFP Gene Plasmid Map 5613
Smad2 cDNA ORF Clone, Mouse, untagged Alternative Names
7120426M23Rik cDNA ORF Clone, Mouse;Madh2 cDNA ORF Clone, Mouse;Madr2 cDNA ORF Clone, Mouse;mMad2 cDNA ORF Clone, Mouse;Smad-2 cDNA ORF Clone, Mouse
Smad2 Background Information

SMAD2 is a member of the SMAD family. Members of this family mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD2 mediates the signal of the TGF-beta, and therefore regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. SMAD2 is the downstream signal transducers of TGF-beta-1 in human dental pulp cells. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. Phosphorylated SMAD2 is able to form a complex with SMAD4 or SARA. These complexes accumulate in the cell nucleus, where they are directly participating in the regulation of gene expression.

Full Name
SMAD family member 2
References
  • Feng. et al., 2002, Mol Cell. 9 (1): 133-43.
  • Zhu Y. et al., 1997, J Biol Chem. 272 (15): 10035-40.
  • Zi Z. et al., 2012, FEBS Lett. 586 (14): 1921-8.
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