Smad5 cDNA ORF Clone, Mouse, N-His tag

Cat: MG50728-NH
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Smad5 cDNA ORF Clone, Mouse, N-His tag 基本信息
基因
种属
Mouse
NCBI 参考序列号
参考序列ORF长度
1398 bp
描述
Full length Clone DNA of Mouse Mothers against decapentaplegic homolog 5 with N terminal His tag.
质粒
启动子
Enhanced CMV promoter
载体
pCMV3-N-His
标签序列
His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

Smad5 cDNA ORF Clone, Mouse, N-His tag Alternative Names
1110051M15Rik cDNA ORF Clone, Mouse;AI451355 cDNA ORF Clone, Mouse;Dwf-C cDNA ORF Clone, Mouse;Madh5 cDNA ORF Clone, Mouse;MusMLP cDNA ORF Clone, Mouse
Smad5 Background Information

SMAD5 is a member of the SMAD family. Members of this family mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD5 is involved in the TGF-beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. It is also involved in cell signalling and modulates signals of bone morphogenetic proteins (BMP's). The binding of ligands causes the oligomerization and phosphorylation of the SMAD5 protein. SMAD5 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase.

Full Name
SMAD family member 5
References
  • Vinayagam A. et al., 2011, Sci Signal. 4 (189): rs8.
  • Sangadala S. et al., 2007, J Biomol Struct Dyn. 25 (1): 11-23.
  • Riggins GJ. et al., 1996, Nat Genet. 13 (3): 347-9.
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