|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|小鼠 TRAIL R2/CD262/TNFRSF10B 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||MG50412-ACG|
|小鼠 TRAIL R2/CD262/TNFRSF10B 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||MG50412-ACR|
|小鼠 TRAIL R2/CD262/TNFRSF10B 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||MG50412-CF|
|小鼠 TRAIL R2/CD262/TNFRSF10B 基因ORF全长cDNA克隆(表达载体), C-His 标签||MG50412-CH|
|小鼠 TRAIL R2/CD262/TNFRSF10B 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||MG50412-CM|
|小鼠 TRAIL R2/CD262/TNFRSF10B 基因ORF全长cDNA克隆(表达载体), C-HA 标签||MG50412-CY|
|小鼠 TRAIL R2/CD262/TNFRSF10B 基因ORF全长cDNA(克隆载体)||MG50412-G|
|小鼠 TRAIL R2/CD262/TNFRSF10B 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||MG50412-NF|
|小鼠 TRAIL R2/CD262/TNFRSF10B 基因ORF全长cDNA克隆(表达载体), N-His 标签||MG50412-NH|
|小鼠 TRAIL R2/CD262/TNFRSF10B 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||MG50412-NM|
|小鼠 TRAIL R2/CD262/TNFRSF10B 基因ORF全长cDNA克隆(表达载体), N-HA 标签||MG50412-NY|
|小鼠 TRAIL R2/CD262/TNFRSF10B 基因ORF全长cDNA克隆(表达载体)||MG50412-UT|
Tumor necrosis factor receptor superfamily, member 10b, official symbol TNFRSF10B, also known as Death receptor 5, CD262, TNF-related apoptosis-inducing ligand receptor 2 (TRAIL R2), is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. TRAIL R2/CD262/TNFRSF10B was purified independently as the only receptor for TRAIL detectable on the surface of two different human cell lines that undergo apoptosis upon stimulation with TRAIL. TRAIL R2/CD262/TNFRSF10B contains two extracellular cysteine-rich repeats, typical for TNF receptor (TNFR) family members, and a cytoplasmic death domain. TRAIL R2/CD262/TNFRSF10B mediates apoptosis via the intracellular adaptor molecule FADD/MORT1. TRAIL receptors can signal both death and gene transcription, functions reminiscent of those of TNFR1 and TRAMP, two other members of the death receptor family. Defects in TRAIL R2/CD262/TNFRSF10B may be a cause of head and neck squamous cell carcinomas (HNSCC) also known as squamous cell carcinoma of the head and neck.