p53 qPCR Primer Pairs, Mouse

Cat: MP200526

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p53 qPCR Primer Pairs, Mouse 基本信息

Target Details

种属:
Mouse

Product Details

Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.

Application & Quality

应用:
SYBR® Green-based quantitative real-time PCR (qPCR).
质控:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.

储存 & 运输

运输:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
储存:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages

Unique Primer Design

To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.

Strict Validation Process

Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.

Uniform PCR conditions, Saving time and cost

~100% amplification curve, ensuring the accuracy of the RNA quantitative

p53 Background Information

p53, also known as Tp53, is a DNA-binding protein which belongs to the p53 family. It contains transcription activation, DNA-binding, and oligomerization domains. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 (TP53) is a transcription factor whose protein levels and post-translational modification state alter in response to cellular stress (such as DNA damage, hypoxia, spindle damage). Activation of p53 begins through a number of mechanisms including phosphorylation by ATM, ATR, Chk1 and MAPKs. MDM2 is a ubiquitn ligase that binds p53 and targets p53 for proteasomal degradation. Phosphorylation, p14ARF and USP7 prevent MDM2-p53 interactions, leading to an increase in stable p53 tetramers in the cytoplasm. Further modifications such as methylation and acetylation lead to an increase in Tp53 binding to gene specific response elements. Tp53 regulates a large number of genes (>100 genes) that control a number of key tumor suppressing functions such as cell cycle arrest, DNA repair, senescence and apoptosis. Whilst the activation of p53 often leads to apoptosis, p53 inactivation facilitates tumor progression. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Defects in TP53 are a cause of esophageal cancer, Li-Fraumeni syndrome, lung cancer and adrenocortical carcinoma.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
tumor protein p53
References
  • Bakhrat A, et al. (2010) Drosophila Chk2 and p53 proteins induce stage-specific cell death independently during oogenesis. Apoptosis. 15(12):1425-34.
  • Kurzhals RL, et al. (2011) Chk2 and p53 are haploinsufficient with dependent and independent functions to eliminate cells after telomere loss. PLoS Genet. 7(6):e1002103.
  • Pardi N, et al. (2011) In vivo effects of abolishing the single canonical sumoylation site in the C-terminal region of Drosophila p53. Acta Biol Hung. 62(4):397-412.
  • Wells BS, et al. (2012) Maintenance of imaginal disc plasticity and regenerative potential in Drosophila by p53. Dev Biol. 361(2):263-76.
  • p53 qPCR Primer Pairs, Cynomolgus, Related Products

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    p53 qPCR Primer Pairs, Mouse, Related Products

    Product Name Catalog#(PDF)
    p53 cDNA ORF Clone in Cloning Vector, Mouse MG50534-M
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    p53 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50534-CF
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    p53 cDNA ORF Clone, Mouse, C-His tag MG50534-CH
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    p53 qPCR Primer Pairs, Human, Related Products

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    p53 cDNA ORF Clone in Cloning Vector, Human HG10182-G
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    p53 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG10182-ACGLN
    p53 cDNA ORF Clone, Human, N-GFPSpark® tag HG10182-ANG
    p53 cDNA ORF Clone, Human, C-OFPSpark® tag HG10182-ACR
    p53 cDNA ORF Clone, Human, N-OFPSpark® tag HG10182-ANR
    p53 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG10182-CF
    p53 cDNA ORF Clone, Human, N-DDK (Flag®) tag HG10182-NF
    p53 cDNA ORF Clone, Human, C-His tag HG10182-CH
    p53 cDNA ORF Clone, Human, N-His tag HG10182-NH
    p53 cDNA ORF Clone, Human, C-HA tag HG10182-CY
    p53 cDNA ORF Clone, Human, N-HA tag HG10182-NY
    p53 cDNA ORF Clone, Human, C-Myc tag HG10182-CM
    p53 cDNA ORF Clone, Human, N-Myc tag HG10182-NM
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