NGF Matched ELISA Antibody Pair Set,Human

Cat: SEK11050
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NGF Matched ELISA Antibody Pair Set,Human General Information
Product name
NGF Matched ELISA Antibody Pair Set,Human
Assay type
Solid Phase Sandwich ELISA
Specificity
Quantitative determination of Human NGF
Assay range
2.81-180
Materials provided
1. Capture Antibody 1 mg/mL of rabbit anti-Human NGF / NGFB monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 11050-RB64)
2. Detection Antibody 0.2 mg/mL of rabbit anti-Human NGF / NGFB monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.25 μg/mL in detection antibody dilution buffer before use. (Catalog: # 11050-R422)
3. Standard Each vial contains 7.7 ng of recombinant Human NGF / NGFB. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 180 pg/mL is recommended.
Product overview
This NGF Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human NGF . It contains Human NGF capture antibody, Human NGF detector antibody (HRP) and a highly purified CHO Stable-expressed recombinant Human NGF protein. This Pair Set is at affordable price for researchers.
Shipping
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
NGF Matched ELISA Antibody Pair Set,Human Images
Human NGF/NGFB/beta-NGF ELISA Pair Set Standard Curve 13905
NGF Matched ELISA Antibody Pair Set,Human Alternative Names
Beta-NGF Matched ELISA Antibody Pair Set, Human;HSAN5 Matched ELISA Antibody Pair Set, Human;NGFB Matched ELISA Antibody Pair Set, Human
NGF Background Information

Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF protein was identified as a large complex consisting of three non-covalently linked subunits, α, β, and γ, among which, the β subunit, called β-NGF (beta-NGF), was demonstrated to exhibits the growth stimulating activity of NGF protein. NGFB/beta-NGF gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. NGF protein acts via at least two receptors on the surface of cells (TrkA and p75 receptors) to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. In addition, previous studies indicated that NGF may also have an important role in the regulation of the immune system.

Full Name
nerve growth factor (beta polypeptide)
References
  • Castellanos MR, et al. (2003) Evaluation of the neurorestorative effects of the murine beta-nerve growth factor infusions in old rat with cognitive deficit. Biochem Biophys Res Commun. 312(4): 867-72.
  • Wang TH, et al. (2008) Effects of pcDNA3-beta-NGF gene-modified BMSC on the rat model of Parkinson's disease. J Mol Neurosci. 35(2): 161-9.
  • Perrard MH, et al. (2009) Redundancy of the effect of TGFbeta1 and beta-NGF on the second meiotic division of rat spermatocytes. Microsc Res Tech. 72(8): 596-602.
  • Product Description Assay Range (pg/ml) Sample Type Catalog# (PDF)
    NGF ELISA Kit, Human Cell supernatant KIT11050
    info info
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