Anti-Nicastrin Antibody

Cat: 11183-R004

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All Nicastrin Reagents

Anti-Nicastrin Antibody (Rabbit Monoclonal antibody) General Information

Product name
Anti-Nicastrin Antibody
Validated applications
Species reactivity
Reacts with: Human
Human Nicastrin
Recombinant Human Nicastrin / NCSTN protein (Catalog#11183-H08H)
This antibody was obtained from a rabbit immunized with purified, recombinant Human Nicastrin / NCSTN (rh Nicastrin / NCSTN; Catalog#11183-H08H; NP_056146.1; Met1-Glu669).
Monoclonal Rabbit IgG Clone #004
Protein A
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Anti-Nicastrin Antibody (Rabbit Monoclonal antibody) Validated Applications

Application Dilution Notes
ICC-IF 1:20-1:100  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Nicastrin Antibody (Rabbit Monoclonal antibody) Images

Human Nicastrin/NCSTN Immunofluorescence(IF) 2881
Immunofluorescence staining of Human NCSTN in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human NCSTN monoclonal antibody (1:60) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

Anti-Nicastrin Antibody Alternative Names

Anti-APH2 Antibody;Anti-ATAG1874 Antibody;Anti-KIAA0253 Antibody;Anti-NCSTN Antibody;Anti-RP11-517F10.1 Antibody

Nicastrin Background Information

Nicastrin (NCST, or NCT), a single-pass membrane glycoprotein that harbors a large extracellular domain, is an essential component of the gamma-secretase complex. Several lines of evidence indicate that the members of these complexes could also contribute to the control of cell death. NCT controls cell death via phosphoinositide 3-kinase/Akt and p53-dependent pathways and that this function remains independent of the activity and molecular integrity of the gamma-secretase complexes. Increasing evidences have shown that Nicastrin/NCSTN plays a crucial role in gamma-cleavage of the amyloid precursor protein (APP). The glycoprotein Nicastrin is an essential component of the gamma-secretase complex, a high molecular weight complex which also contains the presenilin proteins, Aph-1 and Pen-2. The gamma-secretase complex is not only involved in APP processing but also in the processing of an increasing number of other type I integral membrane proteins. As the largest subunit of the gamma-secretase complex, Nicastrin plays a crucial role in its activation. Inhibition of NCSTN demonstrated an altered gamma-cleavage activity, suggesting its potential implication in developing Alzheimer's disease (AD). In addition, Nicastrin can function to maintain epithelial to mesenchymal transition during breast cancer progression. Anti-nicastrin polyclonal and monoclonal antibodies were able to decrease notch1 and vimentin expression and reduced the invasive capacity of breast cancer cells in vitro.

Full Name
  • He G, et al. (2007) Degradation of nicastrin involves both proteasome and lysosome. J Neurochem. 101(4): 982-92.
  • Hayashi I, et al. (2009) Single chain variable fragment against nicastrin inhibits the gamma-secretase activity. J Biol Chem. 284(41): 27838-47.
  • Ma Z, et al. (2009) Association between promoter polymorphisms of the nicastrin gene and sporadic Alzheimer's disease in North Chinese Han population. Neurosci Lett. 458(3): 136-9.
  • Pardossi-Piquard R, et al. (2009) p53-dependent control of cell death by nicastrin: lack of requirement for presenilin-dependent gamma-secretase complex. J Neurochem. 109(1): 225-37.
  • Filipovi? A, et al. (2011) Biological and clinical implications of nicastrin expression in invasive breast cancer. Breast Cancer Res Treat. 125(1): 43-53.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Nicastrin Antibody Rabbit Polyclonal ELISA,IHC-P 50934-T24
    Anti-Nicastrin Antibody Rabbit Polyclonal WB,ELISA,IP 50934-T52
    Anti-Nicastrin Antibody (FITC) Rabbit Monoclonal FCM 11183-R002-F
    Anti-Nicastrin Antibody Rabbit Monoclonal ELISA 11183-R007
    Anti-Nicastrin Antibody Rabbit Polyclonal ELISA 11183-RP01
    Anti-Nicastrin Antibody Rabbit Polyclonal WB,ELISA 11183-RP02
    Anti-Nicastrin Antibody Rabbit Monoclonal FCM 11183-R002
    Anti-Nicastrin Antibody (PE) Rabbit Monoclonal FCM 11183-R002-P
    Anti-Nicastrin Antibody Rabbit Monoclonal WB,IP 11183-R019
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