Anti-Olig2 Antibody

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Anti-Olig2 Antibody (Rabbit Polyclonal 抗体) 产品信息

产品名称
Anti-Olig2 Antibody
经验证的应用
WB,IHC-P,ICC/IF,IF
交叉反应
Reacts with: Human
特异性
Human Olig2
免疫原
A synthetic peptide corresponding to the C-terminus of the Human Olig-2
制备方法
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human Olig-2, and purified by antigen affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Olig2 Antibody (Rabbit Polyclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:1000
IHC-P 1:2500-1:10000
ICC-IF 1:1500-1:50000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Olig2 Antibody (Rabbit Polyclonal 抗体) 图片

Anti-OLIG2 rabbit polyclonal antibody at 1:500 dilution.

Lane A: Mouse heart Tissue lysate Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:32 kDa

Observed band size:30 kDa

(We are unsure as to the identity of these extra bands.)

Immunochemical staining of human OLIG2 in human brain with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).
Immunofluorescence staining of OLIG2 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human OLIG2 polyclonal antibody (dilution ratio 1:5000) at 4℃ overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.

Anti-Olig2 Antibody: 别称

Anti-BHLHB1 Antibody; Anti-bHLHe19 Antibody; Anti-OLIGO2 Antibody; Anti-PRKCBP2 Antibody; Anti-RACK17 Antibody

Olig2 背景信息

Olig2, also known as Oligo2, is a basic helix-loop-helix transcription factor which is expressed in oligodendroglial tumors of the brain. Oligo2 is structurally similar to OLIG1. Both of them have non-overlapping developmental functions in patterning, neuron subtype specification and the formation of oligodendrocytes. Olig2 is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Its over-expression inhibits neural progenitor proliferation through changes in potassium channel activity, thereby contributing to the reduced neuronal numbers and brain size in down syndrome. Olig2 may be a cause of a form of T-cell acute lymphoblastic leukemia.
全称
oligodendrocyte transcription factor 2
参考文献
  • Wang J, et al., 2000, Proc Natl Acad Sci. 97(7): 3497-502.
  • Lu QR, et al., 2001, Proc Natl Acad Sci. 98(19): 10851-6.
  • Marie Y, et al., 2001, Lancet. 358(9278): 298-300.
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