( We provide with PCSK9 qPCR primers for gene expression analysis, HP104404 )
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|人 PCSK9/NARC1 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG15813-ACG|
|人 PCSK9/NARC1 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG15813-ACR|
|人 PCSK9/NARC1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG15813-CF|
|人 PCSK9 ORF mammalian expression plasmid, C-His 标签||HG15813-CH|
|人 PCSK9/NARC1 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG15813-CM|
|人 PCSK9/NARC1 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG15813-CY|
|人 PCSK9/NARC1 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG15813-G-H|
|人 PCSK9/NARC1 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG15813-NF|
|人 PCSK9 ORF mammalian expression plasmid, N-His 标签||HG15813-NH|
|人 PCSK9/NARC1 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG15813-NM|
|人 PCSK9/NARC1 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG15813-NY|
|人 PCSK9/NARC1 基因ORF全长cDNA克隆(表达载体)||HG15813-UT|
Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC1 (neural apoptosis regulated convertase), which is a newly identified human secretory subtilase belonging to the proteinase K subfamily of the secretory subtilase family. PCSK9 protein is an enzyme which in humans is encoded by the PCSK9 gene with orthologs found across many species. It is expressed in neuroepithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. PCSK9 protein is highly expressed in the liver and regulates low density lipoprotein receptor (LDLR) protein levels. Inhibition of PCSK9 protein function is currently being explored as a means of lowering cholesterol levels. Thereby, PCSK9 protein is regarded as a new strategy to treat hypercholesterolemia. PCSK9 protein contributes to cholesterol homeostasis and may have a role in the differentiation of cortical neurons.