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PGDH / PHGDH 抗体, 鼠单抗

产品数据评论相关产品实验方法
Human PHGDH 抗体产品信息
免疫原:Recombinant Human PGDH / PHGDH protein (Catalog#13167-H08E)
克隆号:05
Ig 类型:Mouse IgG2b
浓度:
内毒素:
缓冲液:0.2 μm filtered solution in PBS
抗体制备方法:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PGDH / PHGDH (rh PGDH / PHGDH; Catalog#13167-H08E; O43175; Met1-Phe533). The IgG fraction of the cell culture supernatant was purified by Protein A affinity.
Human PHGDH 抗体使用指导
特异性:Human PGDH / PHGDH
No cross-reactivity in ELISA with
E.coli cell lysate
推荐应用:ELISA

ELISA: 0.5-1 μg/mL

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human PGDH / PHGDH. The detection limit for Human PGDH / PHGDH is approximately 0.078 ng/well.

储存方法:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Other PHGDH 抗体 Products
PGDH/PHGDH 研究背景

PHGDH is a member of the D-isomer specific 2-hydroxyacid dehydrogenase family. This new family consists of D-isomer-stereospecific enzymes. The conserved residues in this family appear to be the residues involved in the substrate binding and the catalytic reaction, and thus to be targets for site-directed mutagenesis. A number of NAD-dependent 2-hydroxyacid dehydrogenases which seem to be specific for the D-isomer of their substrate have been shown to be functionally and structurally related. PHGDH catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor. Overexpression of PHGDH may cause certain breast cancers. Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency which is characterized by congenital microcephaly, psychomotor retardation, and seizures.

Human PGDH/PHGDH 参考资料
  • Pind S, et al. (2002) V490M, a common mutation in 3-phosphoglycerate dehydrogenase deficiency, causes enzyme deficiency by decreasing the yield of mature enzyme. J Biol Chem. 277 (9): 7136-43.
  • Du H, et al. (2010) 3-Phosphoglycerate dehydrogenase expression is regulated by HOXA10 in murine endometrium and human endometrial cells. Reproduction. 139 (1): 237-45.
  • Possemato R, et al. (2011) Functional genomics reveal that the serine synthesis pathway is essential in breast cancer. Nature. 476 (7360): 346-50.
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    货号: 13167-MM05-100
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