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PPM1A / PP2CA / PP2C-alpha 抗体, 鼠单抗

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Human PPM1A 抗体产品信息
免疫原:Recombinant Human PPM1A / PP2C-alpha protein (Catalog#12984-H08E)
克隆号:01
Ig 类型:Mouse IgG2a
浓度:
内毒素:
缓冲液:0.2 μm filtered solution in PBS with 5% trehalose
抗体制备方法:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PPM1A / PP2C-alpha (rh PPM1A / PP2C-alpha; Catalog#12984-H08E; P35813-1; Met 1-Trp 382). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Human PPM1A 抗体使用指导
特异性:Human PPM1A / PP2C-alpha
推荐应用:ICC/IF, IF

ICC/IF: 10-25 μg/mL

储存方法:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human PPM1A 抗体IF实验图片
PPM1A / PP2CA / PP2C-alpha Antibody, Mouse MAb, Immunofluorescence
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Caption:
Immunofluorescence staining of Human PPM1A in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human PPM1A monoclonal antibody (15 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Other PPM1A 抗体 Products
PPM1A/PP2CA 研究背景

Protein phosphatase 1A (PPM1A / PP2CA) is an enzyme belonging to the PP2C family of Ser / Thr protein phosphatases. Members of PP2C family are negative regulators of cell stress response pathways and the MAP kinases and MAP kinase kinases. It has also been demonstrated to inhibit the activation of p38 and JNK kinase cascades. PPM1A dephosphorylates and promotes nuclear export of TGFβ-activated Smad2/3. Ectopic expression of PPM1A abolishes TGFβ-induced antiproliferative and transcriptional responses, whereas depletion of PPM1A enhances TGFβ signaling in mammalian cells. It has been demonstrated that PPM1A / PP2CA, through dephosphorylation of Smad2/3, plays a critical role in terminating TGFβ signaling. Overexpression of PPM1A is reported to activate the expression of the tumor suppressor gene TP53 / p53, which leads to cell apoptosis.

Human PPM1A/PP2CA 参考资料
  • Lin X, et al. (2006) PPM1A functions as a Smad phosphatase to terminate TGFbeta signaling. Cell. 125(5): 915-28.
  • Marc F, et al. (2003) Protein phosphatase 2C binds selectively to and dephosphorylates metabotropic glutamate receptor 3. Proc Natl Acad. 100 (26): 16006-11.
  • Mann DJ, et al. (1992) Mammalian protein serine/threonine phosphatase 2C: cDNA cloning and comparative analysis of amino acid sequences. Biochim Biophys Acta. 1130 (1): 100-4.
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