Anti-PPM1A Antibody (PE)

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Anti-PPM1A Antibody (PE) (Mouse Monoclonal 抗体) 产品信息

产品名称
Anti-PPM1A Antibody (PE)
经验证的应用
FCM
交叉反应
Reacts with: Human
特异性
Human PPM1A
免疫原
Recombinant Human PPM1A / PP2C-alpha protein (Catalog#12984-H08E)
制备方法
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PPM1A / PP2C-alpha (rh PPM1A / PP2C-alpha; Catalog#12984-H08E; P35813-1; Met1-Trp382) and conjugated with PE under optimum conditions, the unreacted PE was removed.
来源
Monoclonal Mouse IgG2a Clone #01
纯化
Protein A
缓冲液
Aqueous solution containing 0.5% BSA and 0.09% sodium azide
偶联物
PE
浓度
10 μl/Test, 0.1 mg/ml
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Anti-PPM1A Antibody (PE) (Mouse Monoclonal 抗体) 图片

Flow cytometric analysis of Human PPM1A expression in Jurkat cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), and then stained with PE Mouse anti-Human PPM1A. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Anti-PPM1A Antibody (PE): 别称

Anti-PP2C-ALPHA Antibody; Anti-PP2CA Antibody; Anti-PP2Calpha Antibody

PPM1A 背景信息

Protein phosphatase 1A (PPM1A / PP2CA) is an enzyme belonging to the PP2C family of Ser / Thr protein phosphatases. Members of PP2C family are negative regulators of cell stress response pathways and the MAP kinases and MAP kinase kinases. It has also been demonstrated to inhibit the activation of p38 and JNK kinase cascades. PPM1A dephosphorylates and promotes nuclear export of TGFβ-activated Smad2/3. Ectopic expression of PPM1A abolishes TGFβ-induced antiproliferative and transcriptional responses, whereas depletion of PPM1A enhances TGFβ signaling in mammalian cells. It has been demonstrated that PPM1A / PP2CA, through dephosphorylation of Smad2/3, plays a critical role in terminating TGFβ signaling. Overexpression of PPM1A is reported to activate the expression of the tumor suppressor gene TP53 / p53, which leads to cell apoptosis.
全称
protein phosphatase, Mg2+/Mn2+ dependent, 1A
参考文献
  • Lin X, et al. (2006) PPM1A functions as a Smad phosphatase to terminate TGFbeta signaling. Cell. 125(5): 915-28.
  • Marc F, et al. (2003) Protein phosphatase 2C binds selectively to and dephosphorylates metabotropic glutamate receptor 3. Proc Natl Acad. 100 (26): 16006-11.
  • Mann DJ, et al. (1992) Mammalian protein serine/threonine phosphatase 2C: cDNA cloning and comparative analysis of amino acid sequences. Biochim Biophys Acta. 1130 (1): 100-4.
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