Ribonuclease A cDNA ORF Clone in Cloning Vector, Human

Cat: HG13468-G
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Ribonuclease A cDNA ORF Clone in Cloning Vector, Human 基本信息
基因
种属
Human
NCBI 参考序列号
参考序列ORF长度
471 bp
序列描述
Identical with the Gene Bank Ref. ID sequence.
描述
Full length Clone DNA of Human ribonuclease, RNase A family, 1 (pancreatic).
质粒
载体
pGEM-T Vector
测序引物
SP6 and T7 or M13-47 and RV-M
质控
The plasmid is confirmed by full-length sequencing.
筛选
抗生素(大肠杆菌)
Ampicillin
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.
Ribonuclease A cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

Ribonuclease A cDNA ORF Clone in Cloning Vector, Human Alternative Names
RAC1 cDNA ORF Clone, Human;RIB1 cDNA ORF Clone, Human;RNS1 cDNA ORF Clone, Human
Ribonuclease A Background Information

RNase A, also known as ribonuclease A and RNASE1, belongs to ribonuclease A superfamily. It is a pancreatic-type of secretory ribonuclease. RNase A is a basic protein and its many positive charges are consistent with its binding to RNA (a poly-anion). More generally, RNase A is unusually polar or, rather, unusually lacking in hydrophobic groups, especially aliphatic ones. As a endonuclease, RNase A cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides, with a pH optimum near 8.0. RNase A is monomeric and more commonly acts to degrade ds-RNA over ss-RNA. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.

Full Name
ribonuclease, RNase A family, 1 (pancreatic)
References
  • Tubert P, et al. (2011) Interactions crucial for three-dimensional domain swapping in the HP-RNase variant PM8. Biophys J. 101(2):459-67.
  • Vinayagam A, et al. (2011) A directed protein interaction network for investigating intracellular signal transduction. Sci Signal. 4(189):rs8.
  • Fischer S, et al. (2011) Expression and localisation of vascular ribonucleases in endothelial cells. Thromb Haemost. 105(2):345-55.
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