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兔 FTL/ferritin, light polypeptide 基因ORF全长cDNA(克隆载体)

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 FTL cDNA Clone产品信息
NCBI注册码:NM_001101688.1
参考序列ORF长度:528bp
cDNA基因描述:Full length Clone DNA of Rabbit ferritin, light polypeptide.
分子别称:FTL2
分子种属:Rabbit
载体:pGEM-T Vector
质粒:pGEM-rabFTL
限制性酶切位点:
Tag序列:
序列信息:Identical with the Gene Bank Ref. ID sequence except for the point mutation: 397C>A(H133N).
测序引物:SP6 and T7 or M13-47 and RV-M
启动子:
Application:
抗生素(大肠杆菌):Ampicillin
抗生素(哺乳动物细胞):
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
兔 FTL/ferritin, light polypeptide 基因ORF全长cDNA(克隆载体) on other vectors
兔 FTL/ferritin, light polypeptide 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签TG65073-ACG1950
兔 FTL/ferritin, light polypeptide 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签TG65073-ACR1950
兔 FTL/ferritin, light polypeptide 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签TG65073-ANG1950
兔 FTL/ferritin, light polypeptide 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签TG65073-ANR1950
兔 FTL/ferritin, light polypeptide 基因ORF全长cDNA克隆(表达载体), C-Flag 标签TG65073-CF1770
兔 FTL/ferritin, light polypeptide 基因ORF全长cDNA克隆(表达载体), C-His 标签TG65073-CH1770
兔 FTL/ferritin, light polypeptide 基因ORF全长cDNA克隆(表达载体), C-Myc 标签TG65073-CM1770
兔 FTL/ferritin, light polypeptide 基因ORF全长cDNA克隆(表达载体), C-HA 标签TG65073-CY1770
兔 FTL/ferritin, light polypeptide 基因ORF全长cDNA(克隆载体)TG65073-G760
兔 FTL/ferritin, light polypeptide 基因ORF全长cDNA克隆(表达载体), N-Flag 标签TG65073-NF1770
兔 FTL/ferritin, light polypeptide 基因ORF全长cDNA克隆(表达载体), N-His 标签TG65073-NH1770
兔 FTL/ferritin, light polypeptide 基因ORF全长cDNA克隆(表达载体), N-Myc 标签TG65073-NM1770
兔 FTL/ferritin, light polypeptide 基因ORF全长cDNA克隆(表达载体), N-HA 标签TG65073-NY1770
兔 FTL/ferritin, light polypeptide 基因ORF全长cDNA克隆(表达载体)TG65073-UT1770
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研究背景

Ferritin, light polypeptide (FTL) is the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Storage of iron in the tissues occurs in the form of ferritin and hemosiderin. The latter originates from ferritin that has undergone intracellular digestion of its protein shell, leaving the iron core. Ferritin and hemosiderin are components of a continuum. Ferritin has been identified in all types of living organisms: animals, plants, molds, and bacteria. Whithin the protein shell of ferritin, iron is first oxidized to the ferric state for storage as ferric oxyhdroxide. Thus, ferritin removes excess iron from the cell sap where it could otherwise participate in peroxidation mechanisms.

参考资料
  • Munro HN, et al. (1988) The ferritin genes: structure, expression, and regulation. Ann N Y Acad Sci. 526: 113-23.
  • Zhang Y, et al. (2008) Comparative proteomic analysis of human placenta derived from assisted reproductive technology. Proteomics. 8 (20): 4344-56.
  • Lebo RV, et al. (1986) Human ferritin light chain gene sequences mapped to several sorted chromosomes. Hum Genet. 71 (4): 325-8.
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    货号: TG65073-G
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