|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|大鼠 ADH5 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||RG81124-ACG|
|大鼠 ADH5 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||RG81124-ACR|
|大鼠 ADH5 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签||RG81124-ANG|
|大鼠 ADH5 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签||RG81124-ANR|
|大鼠 ADH5 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||RG81124-CF|
|大鼠 ADH5 基因ORF全长cDNA克隆(表达载体), C-His 标签||RG81124-CH|
|大鼠 ADH5 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||RG81124-CM|
|大鼠 ADH5 基因ORF全长cDNA克隆(表达载体), C-HA 标签||RG81124-CY|
|大鼠 ADH5 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||RG81124-NF|
|大鼠 ADH5 基因ORF全长cDNA克隆(表达载体), N-His 标签||RG81124-NH|
|大鼠 ADH5 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||RG81124-NM|
|大鼠 ADH5 基因ORF全长cDNA克隆(表达载体), N-HA 标签||RG81124-NY|
|大鼠 ADH5 基因ORF全长cDNA(克隆载体)||RG81124-U|
|大鼠 ADH5 基因ORF全长cDNA克隆(表达载体)||RG81124-UT|
Carbonic anhydrases IX (CAIX), also known as membrane antigen MN or CA9, is a member of the carbonic anhydrase (CA) family and may be involved in cell proliferation and cellular transformation. CAs are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide ( H2O + CO2 = H+ + HCO3- ) and thus participate in a variety of biological and physical processes. CAIX is a transmembrane protein structurally consisting of a signal peptide, a proteoglycan-related region, a CA domain with a highly conserved active site, a transmembrane anchor and an intracytoplasmic tail, and is the only tumor-associated CA isoenzyme known so far. Compared with normal tissues, CAIX is overexpressed in a wide spectrum of tumor types and associated with increased metastasis and poor prognosis in aggressive carcinomas. CAIX expression is cell density dependent and has been shown to be strongly induced by hypoxia, accordingly facilitates adaptation of tumor cells to hypoxic conditions. CA9 is regarded as a new therapeutic target for CA9-derived carcinomas.