ADK qPCR Primer Pairs, Rat

Cat: RP300626
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ADK qPCR Primer Pairs, Rat 基本信息
Target Details
种属:
Rat
Product Details
Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Application & Quality
应用:
SYBR® Green-based quantitative real-time PCR (qPCR).
质控:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
储存 & 运输
运输:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
储存:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages
Unique Primer Design
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Strict Validation Process
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Uniform PCR conditions, Saving time and cost
~100% amplification curve, ensuring the accuracy of the RNA quantitative
ADK Background Information

Adenosine kinase(ADK) belongs to the family of transferases. Adenosine kinase (ADK) is the key enzyme in adenosine metabolism and catalyzes ATP and adenosine into two products: ADP and AMP. Two isoforms of the enzyme adenosine kinase (ADK), which differ at their N-terminal ends, are found in mammalian cells. It has been shown that the two ADK isoforms differ only in their first exons and the promoter regions; hence they arise via differential splicing of their first exons with the other exons common to both isoforms. In adult brain, ADK is primarily present in astrocytes. Several lines of experimental evidence support a critical role of ADK in different types of brain injury associated with astrogliosis, which is also a prominent morphologic feature of temporal lobe epilepsy (TLE). It has been suggested that dysregulation of ADK in astrocytes is a common pathologic hallmark of TLE. Moreover, in vitro data suggest the existence of an additional layer of modulatory crosstalk between the astrocyte-based adenosine cycle and inflammation. ADK also contributes to CK homeostasis in vivo. 

Full Name
adenosine kinase
References
  • Aronica E, et al. (2011) Upregulation of adenosine kinase in astrocytes in experimental and human temporal lobe epilepsy. Epilepsia.52 (9): 1645-55.
  • Kuettel S, et al. (2011) Crystal structures of T. b. rhodesiense adenosine kinase complexed with inhibitor and activator: implications for catalysis and hyperactivation. PLoS Negl Trop Dis. 5 (5): e1164.
  • Cui XA, et al. (2011) Molecular characterization of Chinese hamster cells mutants affected in adenosine kinase and showing novel genetic and biochemical characteristics. BMC Biochem. 12 (1): 22.
  • ADK qPCR Primer Pairs, Rat, Related Products

    Product Name Catalog#(PDF)
    ADK cDNA ORF Clone in Cloning Vector, Rat RG80662-U
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    ADK cDNA ORF Clone, Rat, C-His tag RG80662-CH
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    ADK cDNA ORF Clone, Rat, C-HA tag RG80662-CY
    ADK cDNA ORF Clone, Rat, N-HA tag RG80662-NY
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    ADK qPCR Primer Pairs, Mouse, Related Products

    Product Name Catalog#(PDF)
    ADK cDNA ORF Clone in Cloning Vector, Mouse MG51572-U
    ADK cDNA ORF Clone, Mouse, untagged MG51572-UT
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    ADK cDNA ORF Clone, Mouse, C-GFPSpark® tag MG51572-ACG
    ADK cDNA ORF Clone, Mouse, N-GFPSpark® tag MG51572-ANG
    ADK cDNA ORF Clone, Mouse, C-OFPSpark® tag MG51572-ACR
    ADK cDNA ORF Clone, Mouse, N-OFPSpark® tag MG51572-ANR
    ADK cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG51572-CF
    ADK cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG51572-NF
    ADK cDNA ORF Clone, Mouse, C-His tag MG51572-CH
    ADK cDNA ORF Clone, Mouse, N-His tag MG51572-NH
    ADK cDNA ORF Clone, Mouse, C-HA tag MG51572-CY
    ADK cDNA ORF Clone, Mouse, N-HA tag MG51572-NY
    ADK cDNA ORF Clone, Mouse, C-Myc tag MG51572-CM
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    ADK qPCR Primer Pairs, Human, Related Products

    Product Name Catalog#(PDF)
    ADK cDNA ORF Clone in Cloning Vector, Human HG13149-G
    ADK cDNA ORF Clone, Human, untagged HG13149-UT
    ADK Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG13149-ACGLN
    ADK cDNA ORF Clone, Human, C-GFPSpark® tag HG13149-ACG
    ADK cDNA ORF Clone, Human, N-GFPSpark® tag HG13149-ANG
    ADK cDNA ORF Clone, Human, C-OFPSpark® tag HG13149-ACR
    ADK cDNA ORF Clone, Human, N-OFPSpark® tag HG13149-ANR
    ADK cDNA ORF Clone, Human, C-DDK (Flag®) tag HG13149-CF
    ADK cDNA ORF Clone, Human, N-DDK (Flag®) tag HG13149-NF
    ADK cDNA ORF Clone, Human, C-His tag HG13149-CH
    ADK cDNA ORF Clone, Human, N-His tag HG13149-NH
    ADK cDNA ORF Clone, Human, C-HA tag HG13149-CY
    ADK cDNA ORF Clone, Human, N-HA tag HG13149-NY
    ADK cDNA ORF Clone, Human, C-Myc tag HG13149-CM
    ADK cDNA ORF Clone, Human, N-Myc tag HG13149-NM
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