( We provide with CCR4 qPCR primers for gene expression analysis, RP300327 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|大鼠 CCR4 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||RG80357-ACG|
|大鼠 CCR4 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||RG80357-ACR|
|大鼠 CCR4 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||RG80357-CF|
|大鼠 CCR4 基因ORF全长cDNA克隆(表达载体), C-His 标签||RG80357-CH|
|大鼠 CCR4 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||RG80357-CM|
|大鼠 CCR4 基因ORF全长cDNA克隆(表达载体), C-HA 标签||RG80357-CY|
|大鼠 CCR4 基因ORF全长cDNA(克隆载体)||RG80357-G|
|大鼠 CCR4 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||RG80357-NF|
|大鼠 CCR4 基因ORF全长cDNA克隆(表达载体), N-His 标签||RG80357-NH|
|大鼠 CCR4 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||RG80357-NM|
|大鼠 CCR4 基因ORF全长cDNA克隆(表达载体), N-HA 标签||RG80357-NY|
|大鼠 CCR4 基因ORF全长cDNA克隆(表达载体)||RG80357-UT|
CCR4 is a chemokine receptor that has a critical role in immune cell trafficking. T-helper type 2 cells (Th2), regulatory T cells (Treg), interluekin-17–producing T-helper cells (Th17), and skin-homing memory T cells express CCR4 on their surface and migrate toward the chemokines CCL17 and CCL22. CCR4, a C-C type chemokine receptor, has previously been focused on its biological function in immunopathogenesis of hematological tumors. To date, relatively little attention has been paid to the role of CCR4 in promoting metastasis of some solid tumors, including gastric cancer. Our group has shown that the lymphocyte-rich gastric cancers tend to be more frequently positive for CCR4, and found a novel role of CCR4 in tumor-induced immunosuppression, indicating various expression profiles of CCR4 in gastric cancer tissues and multifunctional roles of this molecule in gastric cancer progression.