|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|大鼠 CD2 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||RG80158-ACG|
|大鼠 CD2 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||RG80158-ACR|
|大鼠 CD2 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||RG80158-CF|
|大鼠 CD2 基因ORF全长cDNA克隆(表达载体), C-His 标签||RG80158-CH|
|大鼠 CD2 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||RG80158-CM|
|大鼠 CD2 基因ORF全长cDNA克隆(表达载体), C-HA 标签||RG80158-CY|
|大鼠 CD2 基因ORF全长cDNA(克隆载体)||RG80158-G|
|大鼠 CD2 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||RG80158-NF|
|大鼠 CD2 基因ORF全长cDNA克隆(表达载体), N-His 标签||RG80158-NH|
|大鼠 CD2 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||RG80158-NM|
|大鼠 CD2 基因ORF全长cDNA克隆(表达载体), N-HA 标签||RG80158-NY|
|大鼠 CD2 基因ORF全长cDNA克隆(表达载体)||RG80158-UT|
T-cell surface antigen CD2, also known as T-cell surface antigen T11/Leu-5, and SRBC, is a single-pass type I membrane protein. It contains one Ig-like C2-type domain and one Ig-like V-type domain. CD2 is a cell adhesion molecule expressed on T cells and is recognized as a target for CD48 (rats) and CD58 (humans). CD2 has been shown to set quantitative thresholds in T cell activation both in vivo and in vitro. Further, intracellular CD2 signaling pathways and networks are being discovered by the identification of several cytosolic tail binding proteins. CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain of CD2 is implicated in the signaling function. The complex of CD2 and CD58 also plays an important role in enhancing the adhesion of T lymphocytes to target cells, and promoting hyperplasia and activation of T lymphocytes. As a cell surface glycoprotein, CD2 expressed on most human T cells and natural killer (NK) cells and plays an important role in mediating cell adhesion in both T-lymphocytes and in signal transduction.