|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|大鼠 PD-L1/B7-H1/CD274 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||RG80450-ACG|
|大鼠 PD-L1/B7-H1/CD274 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||RG80450-ACR|
|大鼠 PD-L1/B7-H1/CD274 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||RG80450-CF|
|大鼠 PD-L1/B7-H1/CD274 基因ORF全长cDNA克隆(表达载体), C-His 标签||RG80450-CH|
|大鼠 PD-L1/B7-H1/CD274 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||RG80450-CM|
|大鼠 PD-L1/B7-H1/CD274 基因ORF全长cDNA克隆(表达载体), C-HA 标签||RG80450-CY|
|大鼠 PD-L1/B7-H1/CD274 基因ORF全长cDNA(克隆载体)||RG80450-G|
|大鼠 PD-L1/B7-H1/CD274 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||RG80450-NF|
|大鼠 PD-L1/B7-H1/CD274 基因ORF全长cDNA克隆(表达载体), N-His 标签||RG80450-NH|
|大鼠 PD-L1/B7-H1/CD274 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||RG80450-NM|
|大鼠 PD-L1/B7-H1/CD274 基因ORF全长cDNA克隆(表达载体), N-HA 标签||RG80450-NY|
|大鼠 PD-L1/B7-H1/CD274 基因ORF全长cDNA克隆(表达载体)||RG80450-UT|
Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1/B7-H1 is a member of the growing B7 family of immune molecules and this protein contains one V-like and one C-like Ig domain within the extracellular domain, and together with PD-L2, are two ligands for PD1 which belongs to the CD28/CTLA4 family expressed on activated lymphoid cells. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.