CD69 qPCR Primer Pairs, Rat

Cat: RP300201

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CD69 qPCR Primer Pairs, Rat 基本信息

Target Details

种属:
Rat

Product Details

Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.

Application & Quality

应用:
SYBR® Green-based quantitative real-time PCR (qPCR).
质控:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.

储存 & 运输

运输:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
储存:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages

Unique Primer Design

To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.

Strict Validation Process

Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.

Uniform PCR conditions, Saving time and cost

~100% amplification curve, ensuring the accuracy of the RNA quantitative

CD69 Background Information

Early activation antigen CD69, also known as activation inducer molecule (AIM), is a single-pass type II membrane protein. Recently, cDNA clones encoding human and mouse CD69 were isolated and showed CD69 to be a member of the C-type lectin superfamily. It is one of the earliest cell surface antigens expressed by T cells following activation. Once expressed, CD69 acts as a costimulatory molecule for T cell activation and proliferation. In addition to mature T cells, CD69 is inducibly expressed by immature thymocytes, B cells, natural killer (NK) cells, monocytes, neutrophils and eosinophils, and is constitutively expressed by mature thymocytes and platelets. CD69 is involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets. The structure, chromosomal localization, expression and function of CD69 suggest that it is likely a pleiotropic immune regulator , potentially important in the activation and differentiation of a wide variety of hematopoietic cells. This membrane molecule transiently expresses on activated lymphocytes, and its selective expression in inflammatory infiltrates suggests that it plays a role in the pathogenesis of inflammatory diseases. CD69 plays a crucial role in the pathogenesis of allergen-induced eosinophilic airway inflammation and hyperresponsiveness and that CD69 could be a possible therapeutic target for asthmatic patients.

Full Name
CD69 molecule
References
  • Ziegler SF, et al. (1994) The activation antigen CD69. Stem Cells. 12(5): 456-65.
  • Marzio R, et al. (1999) CD69 and regulation of the immune function. Immunopharmacol Immunotoxicol. 21(3): 565-82.
  • Lamana A, et al. (2006) The role of CD69 in acute neutrophil-mediated inflammation. Eur J Immunol. 36(10): 2632-8.
  • Miki-Hosokawa T, et al. (2009) CD69 controls the pathogenesis of allergic airway inflammation. J Immunol. 183(12): 8203-15.
  • CD69 qPCR Primer Pairs, Rat, Related Products

    Product Name Catalog#(PDF)
    CD69 cDNA ORF Clone in Cloning Vector, Rat RG80209-G
    CD69 cDNA ORF Clone, Rat, untagged RG80209-UT
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    CD69 Lentiviral cDNA ORF Clone, Rat, C-GFPSpark® tag RG80209-ACGLN
    CD69 cDNA ORF Clone, Rat, C-OFPSpark® tag RG80209-ACR
    CD69 cDNA ORF Clone, Rat, C-DDK (Flag®) tag RG80209-CF
    CD69 cDNA ORF Clone, Rat, N-DDK (Flag®) tag RG80209-NF
    CD69 cDNA ORF Clone, Rat, C-His tag RG80209-CH
    CD69 cDNA ORF Clone, Rat, N-His tag RG80209-NH
    CD69 cDNA ORF Clone, Rat, C-HA tag RG80209-CY
    CD69 cDNA ORF Clone, Rat, N-HA tag RG80209-NY
    CD69 cDNA ORF Clone, Rat, C-Myc tag RG80209-CM
    CD69 cDNA ORF Clone, Rat, N-Myc tag RG80209-NM

    CD69 qPCR Primer Pairs, Mouse, Related Products

    Product Name Catalog#(PDF)
    CD69 cDNA ORF Clone in Cloning Vector, Mouse MG50731-G
    CD69 cDNA ORF Clone, Mouse, untagged MG50731-UT
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    CD69 Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50731-ACGLN
    CD69 cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50731-ACR
    CD69 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50731-CF
    CD69 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50731-NF
    CD69 cDNA ORF Clone, Mouse, C-His tag MG50731-CH
    CD69 cDNA ORF Clone, Mouse, N-His tag MG50731-NH
    CD69 cDNA ORF Clone, Mouse, C-HA tag MG50731-CY
    CD69 cDNA ORF Clone, Mouse, N-HA tag MG50731-NY
    CD69 cDNA ORF Clone, Mouse, C-Myc tag MG50731-CM
    CD69 cDNA ORF Clone, Mouse, N-Myc tag MG50731-NM

    CD69 qPCR Primer Pairs, Human, Related Products

    Product Name Catalog#(PDF)
    CD69 cDNA ORF Clone in Cloning Vector, Human HG11150-M
    CD69 cDNA ORF Clone, Human, untagged HG11150-UT
    CD69 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG11150-ACGLN
    CD69 cDNA ORF Clone, Human, C-GFPSpark® tag HG11150-ACG
    CD69 cDNA ORF Clone, Human, C-OFPSpark® tag HG11150-ACR
    CD69 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG11150-CF
    CD69 cDNA ORF Clone, Human, N-DDK (Flag®) tag HG11150-NF
    CD69 cDNA ORF Clone, Human, C-His tag HG11150-CH
    CD69 cDNA ORF Clone, Human, N-His tag HG11150-NH
    CD69 cDNA ORF Clone, Human, C-HA tag HG11150-CY
    CD69 cDNA ORF Clone, Human, N-HA tag HG11150-NY
    CD69 cDNA ORF Clone, Human, C-Myc tag HG11150-CM
    CD69 cDNA ORF Clone, Human, N-Myc tag HG11150-NM
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