|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|大鼠 CD82/KAI-1 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||RG80332-ACG|
|大鼠 CD82/KAI-1 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||RG80332-ACR|
|大鼠 CD82/KAI-1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||RG80332-CF|
|大鼠 CD82/KAI-1 基因ORF全长cDNA克隆(表达载体), C-His 标签||RG80332-CH|
|大鼠 CD82/KAI-1 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||RG80332-CM|
|大鼠 CD82/KAI-1 基因ORF全长cDNA克隆(表达载体), C-HA 标签||RG80332-CY|
|大鼠 CD82/KAI-1 基因ORF全长cDNA(克隆载体)||RG80332-G|
|大鼠 CD82/KAI-1 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||RG80332-NF|
|大鼠 CD82/KAI-1 基因ORF全长cDNA克隆(表达载体), N-His 标签||RG80332-NH|
|大鼠 CD82/KAI-1 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||RG80332-NM|
|大鼠 CD82/KAI-1 基因ORF全长cDNA克隆(表达载体), N-HA 标签||RG80332-NY|
|大鼠 CD82/KAI-1 基因ORF全长cDNA克隆(表达载体)||RG80332-UT|
CD82, also known as KAI-1, structurally belongs to tetraspanin family while categorised as metastasis suppressor gene on functional grounds. KAI1/CD82 is localized on cell membrane and form interactions with other tetraspanins, integrins and chemokines which are respectively responsible for cell migration, adhesion and signalling. Downregulation of CD82 expression is associated with the advanced stages of many human cancers and correlates with the acquisition of metastatic potential. Recent studies suggest that complex mechanisms underlie CD82 loss of function, including altered transcriptional regulation, splice variant production and post-translational protein modifications, and indicate a central role for CD82 in controlling metastasis as a 'molecular facilitator'. The loss of KAI1/CD82 expression in invasive and metastatic cancers is due to a complex, epigenetic mechanism that probably involves transcription factors such as NFkappaB, p53, and beta-catenin. A loss of KAI1 expression is also associated with the advanced stages of many human malignancies and results in the acquisition of invasive and metastatic capabilities by tumour cells. Thus, KAI1/CD82 is regarded as a wide-spectrum tumor metastasis suppressor.