|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|大鼠 CPA2 基因全长ORF克隆 (表达载体), C端GFPSpark标签||RG80715-ACG|
|大鼠 CPA2 基因全长ORF克隆 (表达载体), C端OFPSpark标签||RG80715-ACR|
|大鼠 CPA2 基因全长ORF克隆 (表达载体), C端FLAG标签||RG80715-CF|
|大鼠 CPA2 基因全长ORF克隆 (表达载体), C端His标签||RG80715-CH|
|大鼠 CPA2 基因全长ORF克隆 (表达载体), C端Myc标签||RG80715-CM|
|大鼠 CPA2 基因全长ORF克隆 (表达载体), C端HA标签||RG80715-CY|
|大鼠 CPA2 基因全长ORF克隆 (表达载体), N端FLAG标签||RG80715-NF|
|大鼠 CPA2 基因全长ORF克隆 (表达载体), N端His标签||RG80715-NH|
|大鼠 CPA2 基因全长ORF克隆 (表达载体), N端Myc标签||RG80715-NM|
|大鼠 CPA2 基因全长ORF克隆 (表达载体), N端HA标签||RG80715-NY|
|大鼠 CPA2 基因全长ORF克隆||RG80715-U|
|大鼠 CPA2 基因全长ORF克隆 (表达载体), 无标签||RG80715-UT|
Human Carboxypeptidase A2 ( CPA2 ) is a secreted pancreatic procarboxy -peptidase, and cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group. The hydrolytic action of CPA2 was identified with a preference towards long substrates with aromatic amino acids in their C-terminal end, particularly tryptophan. CPA2 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. Three different forms of human pancreatic procarboxypeptidase A have been isolated, and the A1 and A2 forms are always secreted as monomeric proteins with different biochemical properties.