|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|大鼠 Fractalkine/CX3CL1 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||RG80163-ACG|
|大鼠 Fractalkine/CX3CL1 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||RG80163-ACR|
|大鼠 Fractalkine/CX3CL1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||RG80163-CF|
|大鼠 Fractalkine/CX3CL1 基因ORF全长cDNA克隆(表达载体), C-His 标签||RG80163-CH|
|大鼠 Fractalkine/CX3CL1 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||RG80163-CM|
|大鼠 Fractalkine/CX3CL1 基因ORF全长cDNA克隆(表达载体), C-HA 标签||RG80163-CY|
|大鼠 Fractalkine/CX3CL1 基因ORF全长cDNA(克隆载体)||RG80163-G|
|大鼠 Fractalkine/CX3CL1 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||RG80163-NF|
|大鼠 Fractalkine/CX3CL1 基因ORF全长cDNA克隆(表达载体), N-His 标签||RG80163-NH|
|大鼠 Fractalkine/CX3CL1 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||RG80163-NM|
|大鼠 Fractalkine/CX3CL1 基因ORF全长cDNA克隆(表达载体), N-HA 标签||RG80163-NY|
|大鼠 Fractalkine/CX3CL1 基因ORF全长cDNA克隆(表达载体)||RG80163-UT|
Fractalkine or Chemokine (C-X3-C motif) ligand 1 (CX3CL1) is a member of the CX3C chemokine family. Fractalkine / CX3CL1 is a unique chemokine that functions not only as a chemoattractant but also as an adhesion molecule and is expressed on endothelial cells activated by proinflammatory cytokines, such as interferon-gamma and tumor necrosis factor-alpha. Fractalkine/CX3CL1 is expressed in a membrane-bound form on activated endothelial cells and mediates attachment and firm adhesion of T cells, monocytes and NK cells. Fractalkine / CX3CL1 is associated with dendritic cells (DC) in epidermis and lymphoid organs. The fractalkine receptor, CX3CR1, is expressed on cytotoxic effector lymphocytes, including natural killer (NK) cells and cytotoxic T lymphocytes, which contain high levels of intracellular perforin and granzyme B, and on macrophages. Soluble fractalkine causes migration of NK cells, cytotoxic T lymphocytes, and macrophages, whereas the membrane-bound form captures and enhances the subsequent migration of these cells in response to secondary stimulation with other chemokines.