( We provide with ECH1 qPCR primers for gene expression analysis, RP300673 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|大鼠 ECH1 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||RG80709-ACG|
|大鼠 ECH1 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||RG80709-ACR|
|大鼠 ECH1 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签||RG80709-ANG|
|大鼠 ECH1 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签||RG80709-ANR|
|大鼠 ECH1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||RG80709-CF|
|大鼠 ECH1 基因ORF全长cDNA克隆(表达载体), C-His 标签||RG80709-CH|
|大鼠 ECH1 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||RG80709-CM|
|大鼠 ECH1 基因ORF全长cDNA克隆(表达载体), C-HA 标签||RG80709-CY|
|大鼠 ECH1 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||RG80709-NF|
|大鼠 ECH1 基因ORF全长cDNA克隆(表达载体), N-His 标签||RG80709-NH|
|大鼠 ECH1 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||RG80709-NM|
|大鼠 ECH1 基因ORF全长cDNA克隆(表达载体), N-HA 标签||RG80709-NY|
|大鼠 ECH1 基因ORF全长cDNA(克隆载体)||RG80709-U|
|大鼠 ECH1 基因ORF全长cDNA克隆(表达载体)||RG80709-UT|
ECH1 is a member of the hydratase/isomerase superfamily. ECH1 shows high sequence similarity to enoyl-CoA hydratases of several species, particularly within a conserved domain characteristic of these proteins. ECH1 contains a C-terminal peroxisomal targeting sequence and localizes to peroxisomes. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans, 5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. ECH1 functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.