( We provide with ENG qPCR primers for gene expression analysis, RP300136 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|大鼠 Endoglin/CD105 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||RG80140-ACG|
|大鼠 Endoglin/CD105 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||RG80140-ACR|
|大鼠 Endoglin/CD105 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||RG80140-CF|
|大鼠 Endoglin/CD105 基因ORF全长cDNA克隆(表达载体), C-His 标签||RG80140-CH|
|大鼠 Endoglin/CD105 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||RG80140-CM|
|大鼠 Endoglin/CD105 基因ORF全长cDNA克隆(表达载体), C-HA 标签||RG80140-CY|
|大鼠 Endoglin/CD105 基因ORF全长cDNA(克隆载体)||RG80140-G|
|大鼠 Endoglin/CD105 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||RG80140-NF|
|大鼠 Endoglin/CD105 基因ORF全长cDNA克隆(表达载体), N-His 标签||RG80140-NH|
|大鼠 Endoglin/CD105 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||RG80140-NM|
|大鼠 Endoglin/CD105 基因ORF全长cDNA克隆(表达载体), N-HA 标签||RG80140-NY|
|大鼠 Endoglin/CD105 基因ORF全长cDNA克隆(表达载体)||RG80140-UT|
Endoglin, also known as CD105, is a type I homodimeric transmembrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated cytoplasmic tail. Endoglin contains an RGD tripeptide which is a key recognition structure in cellular adhesion,,suggesting a critical role for endoglin in the binding of endothelial cells to integrins and/or other RGD receptors. Endoglin is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, mesenchymal stem cells and leukemic cells of lymphoid and myeloid lineages. As an accessory receptor for the TGF-β superfamily ligands, endoglin binds TGF-β1 and TGF-β3 with high affinity not by itself but by associating with TGF-β type I I receptor (TβRII) and activates the downstream signal pathways. In addition, in human umbilical vein endothelial cells, ALK-1 is also a receptor kinase for endoglin threonine phosphorylation, and mutations in either of the two genes result in the autosomal-dominant vascular dysplasia, hereditary hemorrhagic telangiectasia (HHT). Endoglin has been regarded as a powerful biomarker of neovascularization, and is associated with several solid tumor types.