( We provide with FGF9 qPCR primers for gene expression analysis, RP300138 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|大鼠 FGF9 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||RG80143-ACG|
|大鼠 FGF9 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||RG80143-ACR|
|大鼠 FGF9 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||RG80143-CF|
|大鼠 FGF9 基因ORF全长cDNA克隆(表达载体), C-His 标签||RG80143-CH|
|大鼠 FGF9 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||RG80143-CM|
|大鼠 FGF9 基因ORF全长cDNA克隆(表达载体), C-HA 标签||RG80143-CY|
|大鼠 FGF9 基因ORF全长cDNA(克隆载体)||RG80143-G|
|大鼠 FGF9 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||RG80143-NF|
|大鼠 FGF9 基因ORF全长cDNA克隆(表达载体), N-His 标签||RG80143-NH|
|大鼠 FGF9 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||RG80143-NM|
|大鼠 FGF9 基因ORF全长cDNA克隆(表达载体), N-HA 标签||RG80143-NY|
|大鼠 FGF9 基因ORF全长cDNA克隆(表达载体)||RG80143-UT|
Fibroblast growth factor 9 (FGF9) also known as Glia-activating factor or Heparin-binding growth factor 9, is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. Expression of the mouse homolog of this gene was found to be dependent on Sonic hedgehog (Shh) signaling. Mice lacking the homolog gene displayed a male-to-female sex reversal phenotype, which suggested a role in testicular embryogenesis. FGF9 plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. FGF9 may have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.