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大鼠 FTL/ferritin, light polypeptide 基因ORF全长cDNA克隆(表达载体), N-HA 标签

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Rat FTL cDNA Clone产品信息
NCBI注册码:BC061525.1
参考序列ORF长度:552bp
cDNA基因描述:Full length Clone DNA of Rattus norvegicus ferritin, light polypeptide with N terminal HA tag.
分子别称:Ftl1
分子种属:Rat
载体:pCMV3-N-HA
质粒:
限制性酶切位点:
Tag序列:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
序列信息:
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

大鼠 FTL/ferritin, light polypeptide 基因ORF全长cDNA克隆(表达载体), N-HA 标签 on other vectors
大鼠 FTL/ferritin, light polypeptide 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签RG80491-ACG1950
大鼠 FTL/ferritin, light polypeptide 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签RG80491-ACR1950
大鼠 FTL/ferritin, light polypeptide 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签RG80491-ANG1950
大鼠 FTL/ferritin, light polypeptide 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签RG80491-ANR1950
大鼠 FTL/ferritin, light polypeptide 基因ORF全长cDNA克隆(表达载体), C-Flag 标签RG80491-CF1770
大鼠 FTL/ferritin, light polypeptide 基因ORF全长cDNA克隆(表达载体), C-His 标签RG80491-CH1770
大鼠 FTL/ferritin, light polypeptide 基因ORF全长cDNA克隆(表达载体), C-Myc 标签RG80491-CM1770
大鼠 FTL/ferritin, light polypeptide 基因ORF全长cDNA克隆(表达载体), C-HA 标签RG80491-CY1770
大鼠 FTL/ferritin, light polypeptide 基因ORF全长cDNA克隆(表达载体), N-Flag 标签RG80491-NF1770
大鼠 FTL/ferritin, light polypeptide 基因ORF全长cDNA克隆(表达载体), N-His 标签RG80491-NH1770
大鼠 FTL/ferritin, light polypeptide 基因ORF全长cDNA克隆(表达载体), N-Myc 标签RG80491-NM1770
大鼠 FTL/ferritin, light polypeptide 基因ORF全长cDNA克隆(表达载体), N-HA 标签RG80491-NY1770
大鼠 FTL/ferritin, light polypeptide 基因ORF全长cDNA(克隆载体)RG80491-U760
大鼠 FTL/ferritin, light polypeptide 基因ORF全长cDNA克隆(表达载体)RG80491-UT1770
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研究背景

Ferritin, light polypeptide (FTL) is the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Storage of iron in the tissues occurs in the form of ferritin and hemosiderin. The latter originates from ferritin that has undergone intracellular digestion of its protein shell, leaving the iron core. Ferritin and hemosiderin are components of a continuum. Ferritin has been identified in all types of living organisms: animals, plants, molds, and bacteria. Whithin the protein shell of ferritin, iron is first oxidized to the ferric state for storage as ferric oxyhdroxide. Thus, ferritin removes excess iron from the cell sap where it could otherwise participate in peroxidation mechanisms.

参考资料
  • Munro HN, et al. (1988) The ferritin genes: structure, expression, and regulation. Ann N Y Acad Sci. 526: 113-23.
  • Zhang Y, et al. (2008) Comparative proteomic analysis of human placenta derived from assisted reproductive technology. Proteomics. 8 (20): 4344-56.
  • Lebo RV, et al. (1986) Human ferritin light chain gene sequences mapped to several sorted chromosomes. Hum Genet. 71 (4): 325-8.
  • Size / Price
    货号: RG80491-NY
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    库存2-3 weeks
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