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大鼠 Aspartate aminotransferase / GOT1 基因ORF全长cDNA克隆(表达载体), N-HA 标签

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Rat GOT1 cDNA Clone产品信息
NCBI注册码:NM_012571.2
参考序列ORF长度:1242bp
cDNA基因描述:Full length Clone DNA of Rattus norvegicus glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) with N terminal HA tag.
分子别称:cCAT, Aspat, Gaspat, cAspAT
分子种属:Rat
载体:pCMV3-N-HA
质粒:
限制性酶切位点:
Tag序列:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
序列信息:
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

大鼠 Aspartate aminotransferase / GOT1 基因ORF全长cDNA克隆(表达载体), N-HA 标签 on other vectors
大鼠 Aspartate aminotransferase / GOT1 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签RG80493-ACG1950
大鼠 Aspartate aminotransferase / GOT1 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签RG80493-ACR1950
大鼠 Aspartate aminotransferase / GOT1 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签RG80493-ANG1950
大鼠 Aspartate aminotransferase / GOT1 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签RG80493-ANR1950
大鼠 Aspartate aminotransferase / GOT1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签RG80493-CF1770
大鼠 Aspartate aminotransferase / GOT1 基因ORF全长cDNA克隆(表达载体), C-His 标签RG80493-CH1770
大鼠 Aspartate aminotransferase / GOT1 基因ORF全长cDNA克隆(表达载体), C-Myc 标签RG80493-CM1770
大鼠 Aspartate aminotransferase / GOT1 基因ORF全长cDNA克隆(表达载体), C-HA 标签RG80493-CY1770
大鼠 Aspartate aminotransferase / GOT1 基因ORF全长cDNA克隆(表达载体), N-Flag 标签RG80493-NF1770
大鼠 Aspartate aminotransferase / GOT1 基因ORF全长cDNA克隆(表达载体), N-His 标签RG80493-NH1770
大鼠 Aspartate aminotransferase / GOT1 基因ORF全长cDNA克隆(表达载体), N-Myc 标签RG80493-NM1770
大鼠 Aspartate aminotransferase / GOT1 基因ORF全长cDNA克隆(表达载体), N-HA 标签RG80493-NY1770
大鼠 Aspartate aminotransferase / GOT1 基因ORF全长cDNA(克隆载体)RG80493-U760
大鼠 Aspartate aminotransferase / GOT1 基因ORF全长cDNA克隆(表达载体)RG80493-UT1770
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研究背景

Aspartate aminotransferase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, aspartate aminotransferase and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. There is a rare in-frame deletion in aspartate aminotransferase gene, which inactivates cytosolic aspartate aminotransferase(cAST) enzyme in the Old Order Amish. This may help to understand structure and function of the enzyme and would be useful for predicting serum aspartate AST levels.

参考资料
  • Shen H, et al. (2011) Genome-wide association study identifies genetic variants in GOT1 determining serum aspartate aminotransferase levels. J Hum Genet. 56(11):801-5.
  • Doonan S, et al. (1985) Structural and genetic relationships between cytosolic and mitochondrial isoenzymes. Int J Biochem. 16(12):1193-9.
  • Panteghini M. (1990) Aspartate aminotransferase isoenzymes. Clin Biochem. 23(4):311-9.
  • Bousquet-Lemercier B, et al. (1990) Properties of human liver cytosolic aspartate aminotransferase mRNAs generated by alternative polyadenylation site selection. Biochemistry. 29(22):5293-9.
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    货号: RG80493-NY
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