( We provide with KLK13 qPCR primers for gene expression analysis, RP300508 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|大鼠 KLK13/Kallikrein 13 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||RG80544-ACG|
|大鼠 KLK13/Kallikrein 13 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||RG80544-ACR|
|大鼠 KLK13/Kallikrein 13 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||RG80544-CF|
|大鼠 KLK13/Kallikrein 13 基因ORF全长cDNA克隆(表达载体), C-His 标签||RG80544-CH|
|大鼠 KLK13/Kallikrein 13 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||RG80544-CM|
|大鼠 KLK13/Kallikrein 13 基因ORF全长cDNA克隆(表达载体), C-HA 标签||RG80544-CY|
|大鼠 KLK13/Kallikrein 13 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||RG80544-NF|
|大鼠 KLK13/Kallikrein 13 基因ORF全长cDNA克隆(表达载体), N-His 标签||RG80544-NH|
|大鼠 KLK13/Kallikrein 13 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||RG80544-NM|
|大鼠 KLK13/Kallikrein 13 基因ORF全长cDNA克隆(表达载体), N-HA 标签||RG80544-NY|
|大鼠 KLK13/Kallikrein 13 基因ORF全长cDNA(克隆载体)||RG80544-U|
|大鼠 KLK13/Kallikrein 13 基因ORF全长cDNA克隆(表达载体)||RG80544-UT|
Tissue kallikrein 13 (hK13), also known as KLK-L4 (kallikrein-like gene 4), is a member of the human tissue kallikrein family of serine proteases having diverse physiological functions in many tissues. The KLK13 gene resides on chromosome 19q13.3-4 along with other 14 members in a gene cluster and shares a high degree of homology. KLK13 is a trypsin-like, secreted serine protease expressed specifically in the testicular tissue including prostate, salivary gland, breast, and testis. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and may play a role in metastasis. KLK13 may be involved in the pathogenesis and/or progression of breast and ovary cancers, and is regarded as a novel cancer biomarker. In addition, KLK13 interacts and forms complexes with several serum protease inhibitors, such as alpha2-macroglobulin, and its expression is regulated by steroid hormones.