|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|大鼠 LTBR/TNFRSF3 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||RG80178-ACG|
|大鼠 LTBR/TNFRSF3 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||RG80178-ACR|
|大鼠 LTBR/TNFRSF3 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||RG80178-CF|
|大鼠 LTBR/TNFRSF3 基因ORF全长cDNA克隆(表达载体), C-His 标签||RG80178-CH|
|大鼠 LTBR/TNFRSF3 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||RG80178-CM|
|大鼠 LTBR/TNFRSF3 基因ORF全长cDNA克隆(表达载体), C-HA 标签||RG80178-CY|
|大鼠 LTBR/TNFRSF3 基因ORF全长cDNA(克隆载体)||RG80178-G|
|大鼠 LTBR/TNFRSF3 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||RG80178-NF|
|大鼠 LTBR/TNFRSF3 基因ORF全长cDNA克隆(表达载体), N-His 标签||RG80178-NH|
|大鼠 LTBR/TNFRSF3 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||RG80178-NM|
|大鼠 LTBR/TNFRSF3 基因ORF全长cDNA克隆(表达载体), N-HA 标签||RG80178-NY|
|大鼠 LTBR/TNFRSF3 基因ORF全长cDNA克隆(表达载体)||RG80178-UT|
LTBR (lymphotoxin beta receptor (TNFR superfamily, member 3)) is a member of the tumor necrosis factor (TNF) family of receptors. Tumor necrosis factor receptor is a trimeric cytokine receptor that binds tumor necrosis factors. The receptor cooperates with an adaptor protein (such as TRADD, TRAF, RIP), which is important in determining the outcome of the response. LTBR is expressed on the surface of most cell types, including cells of epithelial and myeloid lineages, but not on T and B lymphocytes. LTBR specifically binds the lymphotoxin membrane form (a complex of lymphotoxin-alpha and lymphtoxin-beta). LTBR and its ligand play a role in the development and organization of lymphoid tissue and tranformed cells. Activation of this protein can trigger apoptosis. Not only does the LTBR help trigger apoptosis, it can lead to the release of the cytokine interleukin 8. Overexpression of LTBR in HEK293 cells increases IL-8 promoter activity and leads to IL-8 release. It is also essential for development and organization of the secondary lymphoid organs and chemokine release.