( We provide with NCSTN qPCR primers for gene expression analysis, RP301007 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.
|大鼠 Nicastrin/NCSTN 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||RG81043-ACG|
|大鼠 Nicastrin/NCSTN 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||RG81043-ACR|
|大鼠 Nicastrin/NCSTN 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||RG81043-CF|
|大鼠 Nicastrin/NCSTN 基因ORF全长cDNA克隆(表达载体), C-His 标签||RG81043-CH|
|大鼠 Nicastrin/NCSTN 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||RG81043-CM|
|大鼠 Nicastrin/NCSTN 基因ORF全长cDNA克隆(表达载体), C-HA 标签||RG81043-CY|
|大鼠 Nicastrin/NCSTN 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||RG81043-NF|
|大鼠 Nicastrin/NCSTN 基因ORF全长cDNA克隆(表达载体), N-His 标签||RG81043-NH|
|大鼠 Nicastrin/NCSTN 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||RG81043-NM|
|大鼠 Nicastrin/NCSTN 基因ORF全长cDNA克隆(表达载体), N-HA 标签||RG81043-NY|
|大鼠 Nicastrin/NCSTN 基因ORF全长cDNA(克隆载体)||RG81043-U|
|大鼠 Nicastrin/NCSTN 基因ORF全长cDNA克隆(表达载体)||RG81043-UT|
Nicastrin (NCST, or NCT), a single-pass membrane glycoprotein that harbors a large extracellular domain, is an essential component of the gamma-secretase complex. Several lines of evidence indicate that the members of these complexes could also contribute to the control of cell death. NCT controls cell death via phosphoinositide 3-kinase/Akt and p53-dependent pathways and that this function remains independent of the activity and molecular integrity of the gamma-secretase complexes. Increasing evidences have shown that Nicastrin/NCSTN plays a crucial role in gamma-cleavage of the amyloid precursor protein (APP). The glycoprotein Nicastrin is an essential component of the gamma-secretase complex, a high molecular weight complex which also contains the presenilin proteins, Aph-1 and Pen-2. The gamma-secretase complex is not only involved in APP processing but also in the processing of an increasing number of other type I integral membrane proteins. As the largest subunit of the gamma-secretase complex, Nicastrin plays a crucial role in its activation. Inhibition of NCSTN demonstrated an altered gamma-cleavage activity, suggesting its potential implication in developing Alzheimer's disease (AD). In addition, Nicastrin can function to maintain epithelial to mesenchymal transition during breast cancer progression. Anti-nicastrin polyclonal and monoclonal antibodies were able to decrease notch1 and vimentin expression and reduced the invasive capacity of breast cancer cells in vitro.