快速订购

大鼠 ADP-sugar Pyrophosphatase/NUDT5 基因ORF全长cDNA克隆(表达载体), C-Myc 标签

产品数据评论相关产品实验方法
大鼠 NUDT5 cDNA Clone产品信息
NCBI注册码:NM_001007733.1
参考序列ORF长度:660bp
cDNA基因描述:Full length Clone DNA of Rattus norvegicus nudix (nucleoside diphosphate linked moiety X)-type motif 5 with C terminal Myc tag.
分子别称:Nudt5
分子种属:Rat
载体:pCMV3-C-Myc
质粒:
限制性酶切位点:
Tag序列:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
序列信息:
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
( We provide with NUDT5 qPCR primers for gene expression analysis, RP301066 )
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

大鼠 ADP-sugar Pyrophosphatase/NUDT5 基因ORF全长cDNA克隆(表达载体), C-Myc 标签 on other vectors
大鼠 ADP-sugar Pyrophosphatase/NUDT5 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签RG81109-ACG1950
大鼠 ADP-sugar Pyrophosphatase/NUDT5 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签RG81109-ACR1950
大鼠 ADP-sugar Pyrophosphatase/NUDT5 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签RG81109-ANG1950
大鼠 ADP-sugar Pyrophosphatase/NUDT5 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签RG81109-ANR1950
大鼠 ADP-sugar Pyrophosphatase/NUDT5 基因ORF全长cDNA克隆(表达载体), C-Flag 标签RG81109-CF1770
大鼠 ADP-sugar Pyrophosphatase/NUDT5 基因ORF全长cDNA克隆(表达载体), C-His 标签RG81109-CH1770
大鼠 ADP-sugar Pyrophosphatase/NUDT5 基因ORF全长cDNA克隆(表达载体), C-Myc 标签RG81109-CM1770
大鼠 ADP-sugar Pyrophosphatase/NUDT5 基因ORF全长cDNA克隆(表达载体), C-HA 标签RG81109-CY1770
大鼠 ADP-sugar Pyrophosphatase/NUDT5 基因ORF全长cDNA克隆(表达载体), N-Flag 标签RG81109-NF1770
大鼠 ADP-sugar Pyrophosphatase/NUDT5 基因ORF全长cDNA克隆(表达载体), N-His 标签RG81109-NH1770
大鼠 ADP-sugar Pyrophosphatase/NUDT5 基因ORF全长cDNA克隆(表达载体), N-Myc 标签RG81109-NM1770
大鼠 ADP-sugar Pyrophosphatase/NUDT5 基因ORF全长cDNA克隆(表达载体), N-HA 标签RG81109-NY1770
大鼠 ADP-sugar Pyrophosphatase/NUDT5 基因ORF全长cDNA(克隆载体)RG81109-U760
大鼠 ADP-sugar Pyrophosphatase/NUDT5 基因ORF全长cDNA克隆(表达载体)RG81109-UT1770
 点击了解更多载体信息
Product nameProduct name
研究背景

ADP-sugar Pyrophosphatase, also known as NUDT5, eliminates toxic nucleotide derivatives from the cell and regulate the levels of important signaling nucleotides and their metabolites. NUDT5 functions as a MutT-related protein and catalyzes the hydrolysis of 8-oxoGDP to 8-oxoGMP, thereby preventing misincorporation of 8-oxoGua into RNA. NUDT5 may play significant roles in regulating the G1-S transition in mammalian cells. It can also hydrolyze other nucleotide sugars with low activity.

参考资料
  • Ishibashi T. et al., 2004, EMBO Rep. 4 (5): 479-8.
  • Gerhard DS. et al., 2004, Genome Res. 14 (10B): 2121-7.
  • Rush J. et al., 2005, Nat Biotechnol. 23 (1): 94-101.
  • Kamiya H. et al., 2009, DNA Repair. 8 (10): 1250-4.
  • Size / Price
    货号: RG81109-CM
    目录价: 
    单价:      (You Save: )
    Contact Us
    产品图片
        产品数据可能随批次变化,最新数据请详见随货说明书。
        请注意:所有产品都是“仅用于科研,而不能用于诊断或治疗用途”