|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|大鼠 NUDT5 基因全长ORF克隆 (表达载体), C端GFPSpark标签||RG81109-ACG|
|大鼠 NUDT5 基因全长ORF克隆 (表达载体), C端OFPSpark标签||RG81109-ACR|
|大鼠 NUDT5 基因全长ORF克隆 (表达载体), N端GFPSpark标签||RG81109-ANG|
|大鼠 NUDT5 基因全长ORF克隆 (表达载体), N端OFPSpark标签||RG81109-ANR|
|大鼠 NUDT5 基因全长ORF克隆 (表达载体), C端FLAG标签||RG81109-CF|
|大鼠 NUDT5 基因全长ORF克隆 (表达载体), C端His标签||RG81109-CH|
|大鼠 NUDT5 基因全长ORF克隆 (表达载体), C端Myc标签||RG81109-CM|
|大鼠 NUDT5 基因全长ORF克隆 (表达载体), C端HA标签||RG81109-CY|
|大鼠 NUDT5 基因全长ORF克隆 (表达载体), N端FLAG标签||RG81109-NF|
|大鼠 NUDT5 基因全长ORF克隆 (表达载体), N端His标签||RG81109-NH|
|大鼠 NUDT5 基因全长ORF克隆 (表达载体), N端Myc标签||RG81109-NM|
|大鼠 NUDT5 基因全长ORF克隆 (表达载体), N端HA标签||RG81109-NY|
|大鼠 NUDT5 基因全长ORF克隆||RG81109-U|
|大鼠 NUDT5 基因全长ORF克隆 (表达载体), 无标签||RG81109-UT|
ADP-sugar Pyrophosphatase, also known as NUDT5, eliminates toxic nucleotide derivatives from the cell and regulate the levels of important signaling nucleotides and their metabolites. NUDT5 functions as a MutT-related protein and catalyzes the hydrolysis of 8-oxoGDP to 8-oxoGMP, thereby preventing misincorporation of 8-oxoGua into RNA. NUDT5 may play significant roles in regulating the G1-S transition in mammalian cells. It can also hydrolyze other nucleotide sugars with low activity.