NUDT5 cDNA ORF Clone, Rat, C-Myc tag

Cat: RG81109-CM
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NUDT5 cDNA ORF Clone, Rat, C-Myc tag 基本信息
基因
种属
Rat
NCBI 参考序列号
参考序列ORF长度
660 bp
描述
Full length Clone DNA of Rat nudix (nucleoside diphosphate linked moiety X)-type motif 5 with C terminal Myc tag.
质粒
启动子
Enhanced CMV promoter
载体
pCMV3-C-Myc
标签序列
Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

NUDT5 Background Information

ADP-sugar Pyrophosphatase, also known as NUDT5, eliminates toxic nucleotide derivatives from the cell and regulate the levels of important signaling nucleotides and their metabolites. NUDT5 functions as a MutT-related protein and catalyzes the hydrolysis of 8-oxoGDP to 8-oxoGMP, thereby preventing misincorporation of 8-oxoGua into RNA. NUDT5 may play significant roles in regulating the G1-S transition in mammalian cells. It can also hydrolyze other nucleotide sugars with low activity.

Full Name
nudix (nucleoside diphosphate linked moiety X)-type motif 5
References
  • Ishibashi T. et al., 2004, EMBO Rep. 4 (5): 479-8.
  • Gerhard DS. et al., 2004, Genome Res. 14 (10B): 2121-7.
  • Rush J. et al., 2005, Nat Biotechnol. 23 (1): 94-101.
  • Kamiya H. et al., 2009, DNA Repair. 8 (10): 1250-4.
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