|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|大鼠 PD1/PDCD1/CD279 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||RG80448-ACG|
|大鼠 PD1/PDCD1/CD279 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||RG80448-ACR|
|大鼠 PD1/PDCD1/CD279 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||RG80448-CF|
|大鼠 PD1/PDCD1/CD279 基因ORF全长cDNA克隆(表达载体), C-His 标签||RG80448-CH|
|大鼠 PD1/PDCD1/CD279 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||RG80448-CM|
|大鼠 PD1/PDCD1/CD279 基因ORF全长cDNA克隆(表达载体), C-HA 标签||RG80448-CY|
|大鼠 PD1/PDCD1/CD279 基因ORF全长cDNA(克隆载体)||RG80448-G|
|大鼠 PD1/PDCD1/CD279 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||RG80448-NF|
|大鼠 PD1/PDCD1/CD279 基因ORF全长cDNA克隆(表达载体), N-His 标签||RG80448-NH|
|大鼠 PD1/PDCD1/CD279 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||RG80448-NM|
|大鼠 PD1/PDCD1/CD279 基因ORF全长cDNA克隆(表达载体), N-HA 标签||RG80448-NY|
|大鼠 PD1/PDCD1/CD279 基因ORF全长cDNA克隆(表达载体)||RG80448-UT|
Programmed cell death 1, also known as PDCD1, is a type I transmembrane glycoprotein, and is an immunoreceptor belonging to the CD28/CTLA-4 family negatively regulates antigen receptor signaling by recruiting protein tyrosine phosphatase, SHP-2 upon interacting with either of two ligands, PD-L1 or PD-L2. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. PD1 has been suggested to be involved in lymphocyte clonal selection and peripheral tolerance, and thus contributes to the prevention of autoimmune diseases. Furthermore, PD1 is shown to be a regulator of virus-specific CD8+ T cell survival in HIV infection. As a cell surface molecule, PDCD1 regulates the adaptive immune response. Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function.