|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|大鼠 Podoplanin 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||RG81154-ACG|
|大鼠 Podoplanin 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||RG81154-ACR|
|大鼠 Podoplanin 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||RG81154-CF|
|大鼠 Podoplanin 基因ORF全长cDNA克隆(表达载体), C-His 标签||RG81154-CH|
|大鼠 Podoplanin 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||RG81154-CM|
|大鼠 Podoplanin 基因ORF全长cDNA克隆(表达载体), C-HA 标签||RG81154-CY|
|大鼠 Podoplanin 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||RG81154-NF|
|大鼠 Podoplanin 基因ORF全长cDNA克隆(表达载体), N-His 标签||RG81154-NH|
|大鼠 Podoplanin 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||RG81154-NM|
|大鼠 Podoplanin 基因ORF全长cDNA克隆(表达载体), N-HA 标签||RG81154-NY|
|大鼠 Podoplanin 基因ORF全长cDNA(克隆载体)||RG81154-U|
|大鼠 Podoplanin 基因ORF全长cDNA克隆(表达载体)||RG81154-UT|
Podoplanin, also known as PDPN, is a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character. The homologous protein in other species has been described as a differentiation antigen and influenza-virus receptor. The specific function of this protein has not been determined. Alternatively spliced transcript variants encoding different isoforms have been identified.PDPN is a mucin-type glycoprotein negatively charged by extensive O-glycosylation and a high content of sialic acid, which expresses the adhesive property. It is selectively expressed in lymphatic endothelium as well as lymphangiomas, Kaposi sarcomas, and in a subset of angiosarcomas with probable lymphatic differentiation. PDPN may contribute to form odontoblastic fiber or function as the anchorage to the tooth development and in proliferating epithelial cells of cervical loop and apical bud. The intensity of podoplanin expression is negatively correlated with the expression of CD34 and factor VIII. Podoplanin would be useful as a diagnostic marker for epithelioid hemangioendothelioma in liver tumors.