( We provide with PTMA qPCR primers for gene expression analysis, RP301128 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|大鼠 PTMA 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||RG81172-ACG|
|大鼠 PTMA 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||RG81172-ACR|
|大鼠 PTMA 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签||RG81172-ANG|
|大鼠 PTMA 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签||RG81172-ANR|
|大鼠 PTMA 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||RG81172-CF|
|大鼠 PTMA 基因ORF全长cDNA克隆(表达载体), C-His 标签||RG81172-CH|
|大鼠 PTMA 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||RG81172-CM|
|大鼠 PTMA 基因ORF全长cDNA克隆(表达载体), C-HA 标签||RG81172-CY|
|大鼠 PTMA 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||RG81172-NF|
|大鼠 PTMA 基因ORF全长cDNA克隆(表达载体), N-His 标签||RG81172-NH|
|大鼠 PTMA 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||RG81172-NM|
|大鼠 PTMA 基因ORF全长cDNA克隆(表达载体), N-HA 标签||RG81172-NY|
|大鼠 PTMA 基因ORF全长cDNA(克隆载体)||RG81172-U|
|大鼠 PTMA 基因ORF全长cDNA克隆(表达载体)||RG81172-UT|
PTMA (prothymosin, alpha, N-GST chimera) is a small, 12.4 kDa protein. It is a 109-111 amino acid long polypeptide as the precursor of thymosin a1. Thymosins are named becaues they were originally isolated from the thymus. But now in many other tissues, thymosins also can be detected. Thymosins have diverse biological activities, and two in particular, thymosins a1 and _4, have potentially important uses in medicine, some of which have already progressed from the laboratory to the clinic. In general, PTMA is associated with cellular proliferation and carcinogenesis (Eschenfeldt et al., 1986), cellular and viral transcription (Cotter et al., 2000), protection against apoptosis and chromatin remodelling (Karetsou et al., 1998). PTMA may have a dual role both intracellulary and extracellulary. In relation to diseases, thymosins have been categorized as biological response modifiers. Thymosin a1 is derived from PTMA. For animals that lack thymus glands, thymosin a1 is responsible for the activity of that preparation in restoring immune function.