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大鼠 PTN 基因全长ORF克隆 (表达载体), C端Myc标签

产品数据评论相关产品实验方法
Rat PTN cDNA Clone产品信息
NCBI注册码:NM_017066.2
参考序列ORF长度:507bp
cDNA基因描述:Full length Clone DNA of Rattus norvegicus pleiotrophin with C terminal Myc tag.
分子别称:Hbnf
分子种属:Rat
载体:pCMV3-C-Myc
质粒:
限制性酶切位点:
Tag序列:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
序列信息:
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Product nameProduct name
Human MANF / ARMET Protein (Fc Tag)重组人 NTF3 / Neurotrophin 3 蛋白重组人 CDNF / ARMETL1 蛋白 (Fc 标签)重组狗 TrkB / NTRK2 蛋白 (Fc 标签)Rabbit TrkA / NTRK1 Protein (His Tag)Rabbit NGFR / TNFRSF16 / P75 Protein (ECD, His Tag)重组人 TrkB / NTRK2 蛋白 (His & Fc 标签)重组人 TrkB / NTRK2 蛋白 (His 标签)重组人 TrkC / NTRK3 蛋白 (His & Fc 标签)重组人 TrkC / NTRK3 蛋白 (His 标签)重组人 CDNF / ARMETL1 蛋白 (His 标签)重组人 β-NGF / Beta-NGF 蛋白重组人 TrkA / NTRK1 蛋白 (His & Fc 标签)重组人 TrkA / NTRK1 蛋白 (aa 285-413, His 标签)重组人 TrkA / NTRK1 蛋白 (aa 194-413, His 标签)重组人 TrkA / NTRK1 蛋白 (His 标签)重组人 MANF / ARMET 蛋白 (His 标签)重组人 NGFR / P75 蛋白 (Fc 标签)重组人 NGFR/ P75 蛋白 (His 标签)重组小鼠 TrkB / NTRK2 蛋白 (His 标签)重组小鼠 / 人 BDNF 蛋白重组小鼠 TrkC / NTRK3 蛋白 (His 标签)重组小鼠 β-NGF / Beta-NGF 蛋白重组小鼠 CDNF / ARMETL1 蛋白 (His 标签)重组小鼠 MANF / ARMET 蛋白 (His 标签)重组小鼠 NGFR / P75 蛋白 (Fc 标签)重组小鼠 NGFR / P75 蛋白 (His 标签)重组小鼠 TrkA / NTRK1 蛋白 (Fc 标签)重组小鼠 TrkA / NTRK1 蛋白 (His 标签)重组狗 TrkB / NTRK2 蛋白 (His 标签)重组狗 TrkA / NTRK1 蛋白 (Fc 标签)重组狗 TrkA / NTRK1 蛋白 (His 标签)重组大鼠 TrkB / NTRK2 蛋白 (Fc 标签)重组大鼠 TrkB / NTRK2 蛋白 (His 标签)重组大鼠 TrkA / NTRK1 蛋白 (Fc 标签)重组大鼠 TrkA / NTRK1 蛋白 (His 标签)
研究背景

HB-GAM belongs to the pleiotrophin family. During embryonic and early postnatal development, HB-GAM is expressed in the central and peripheral nervous system and also in several non-neural tissues, notably lung, kidney, gut and bone. While in the adult central nervous system, it is expressed in an activity-dependent manner in the hippocampus where it can suppress long term potentiation induction. HB-GAM has a low expression in other areas of the adult brain, but it can be induced by ischemic insults, or targeted neuronal damaged in the entorhinal cortex or in the substantia nigra pars compacta. It is structurally related to midkine and retinoic acid induced heparin-binding protein and has a high affinity for heparin. HB-GAM binds anaplastic lymphoma kinase (ALK) which induces MAPK pathway activation, an important step in the anti-apoptotic signaling of PTN and regulation of cell proliferation. It also functions as a secreted growth factor and induces neurite outgrowth and which is mitogenic for fibroblasts, epithelial, and endothelial cells.

参考资料
  • Vanderwinden JM, et al. (1992) Cellular distribution of the new growth factor pleiotrophin (HB-GAM) mRNA in developing and adult rat tissues. Anat Embryol. 186(4):387-406.
  • Lauri SE, et al. (1996) Activity-induced enhancement of HB-GAM expression in rat hippocampal slices. Neuroreport. 7(10):1670-4.
  • Pavlov I, et al. (2002) Role of heparin-binding growth-associated molecule (HB-GAM) in hippocampal LTP and spatial learning revealed by studies on overexpressing and knockout mice. Mol Cell Neurosci. 20(2):330-42.
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