RRM1 cDNA ORF Clone, Rat, C-His tag

Cat: RG81060-CH
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RRM1 cDNA ORF Clone, Rat, C-His tag 基本信息
基因
种属
Rat
NCBI 参考序列号
参考序列ORF长度
2379 bp
描述
Full length Clone DNA of Rat ribonucleotide reductase M1 with C terminal His tag.
质粒
启动子
Enhanced CMV promoter
载体
pCMV3-C-His
标签序列
His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

RRM1 Background Information

RRM1 is a subunit of ribonucleoside-diphosphate reductase which is constituted by two subunits. Ribonucleoside-diphosphate reductase is an enzyme essential for the production of deoxyribonucleotides prior to DNA synthesis in S phase of dividing cells. RRM1 is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. RRM1 may play a role in malignancies and disease that involve this region.

Full Name
ribonucleotide reductase M1
References
  • Pitterle DM, et al. (1999) Human gene for the large subunit of ribonucleotide reductase (RRM1): functional analysis of the promoter. Genomics. 27(2):280-5.
  • Parker NJ, et al. (1995) Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc Natl Acad Sci. 99(26):16899-903.
  • Gautam A, et al. (2003) RRM1-induced metastasis suppression through PTEN-regulated pathways. Oncogene. 22(14):2135-42.
  • RRM1 cDNA ORF Clone, Rat, Related Products

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    RRM1 cDNA ORF Clone, Human, Related Products

    Product Name Catalog#(PDF)
    RRM1 cDNA ORF Clone in Cloning Vector, Human HG14316-G
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    RRM1 cDNA ORF Clone, Human, C-GFPSpark® tag HG14316-ACG
    RRM1 cDNA ORF Clone, Human, N-GFPSpark® tag HG14316-ANG
    RRM1 cDNA ORF Clone, Human, C-OFPSpark® tag HG14316-ACR
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    RRM1 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG14316-CF
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    RRM1 cDNA ORF Clone, Human, C-His tag HG14316-CH
    RRM1 cDNA ORF Clone, Human, N-His tag HG14316-NH
    RRM1 cDNA ORF Clone, Human, C-HA tag HG14316-CY
    RRM1 cDNA ORF Clone, Human, N-HA tag HG14316-NY
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    RRM1 cDNA ORF Clone, Human, N-Myc tag HG14316-NM
    RRM1 qPCR Primer Pairs, Human HP102966
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