PAI-1 cDNA ORF Clone, Rat, N-HA tag

Cat: RG80442-NY
Contact us:
215-583-7898
order@sinobiological.com
Distributors
All PAI-1 Reagents
CRO Services
Product recommended

All PAI-1 Reagents
PAI-1 cDNA ORF Clone, Rat, N-HA tag 基本信息
基因
种属
Rat
NCBI 参考序列号
参考序列ORF长度
1209 bp
描述
Full length Clone DNA of Rat serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 with N terminal HA tag.
质粒
启动子
Enhanced CMV promoter
载体
pCMV3-SP-N-HA
标签序列
HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

PAI-1 cDNA ORF Clone, Rat, N-HA tag Alternative Names
Pai1 cDNA ORF Clone, Rat;Planh1 cDNA ORF Clone, Rat;SERPINE1 cDNA ORF Clone, Rat
PAI-1 Background Information

Plasminogen activator inhibitor 1, also known as PAI-1, Endothelial plasminogen activator inhibitor, SerpinE1 and PLANH1, is a secreted glycoprotein which belongs to the serpin family. SerpinE1 is the primary physiological inhibitor of the two plasminogen activators urokinase (uPA) and tissue plasminogen activator (tPA). Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. Defects in SerpinE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency) which is characterized by abnormal bleeding due to SerpinE1 defect in the plasma. High concentrations of SerpinE1 have been associated with thrombophilia which is an autosomal dominant disorder in which affected individuals are prone to develop serious spontaneous thrombosis. Studies of PAI-1 have contributed significantly to the elucidation of the protease inhibitory mechanism of serpins, which is based on a metastable native state becoming stabilised by insertion of the RCL into the central beta-sheet A and formation of covalent complexes with target proteases. Greater expression of PAI-1 has been associated with increased survival of cells and resistance to apoptosis. PAI-1 appears to influence apoptosis by decreasing cell adhesion (anoikis) as well as its effect on intracellular signaling. PAI-1, in its active state, also binds to the extracellular protein vitronectin. When in complex with its target proteases, it binds with high affinity to endocytosis receptors of the low density receptor family. The mechanisms of PAI-1 overexpression during obesity are complex, and it is conceivable that several inducers are involved at the same time at several sites of synthesis. PAI-1 is also implicated in adipose tissue development. It suggests that PAI-1 inhibitors serve in the control of atherothrombosis.

Full Name
serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
References
  • Alessi MC, et al. (2006) PAI-1 and the metabolic syndrome: links, causes, and consequences. Arterioscler Thromb Vasc Biol. 26(10): 2200-7.
  • Schneider DJ, et al. (2008) The effect of plasminogen activator inhibitor type 1 on apoptosis. Thromb Haemost. 100(6): 1037-40.
  • Dupont DM, et al. (2009) Biochemical properties of plasminogen activator inhibitor-1. Front Biosci. 14: 1337-61.
  • PAI-1 cDNA ORF Clone, Rat, Related Products

    Product Name Catalog#(PDF)
    PAI-1 cDNA ORF Clone in Cloning Vector, Rat RG80442-G
    PAI-1 cDNA ORF Clone, Rat, untagged RG80442-UT
    PAI-1 cDNA ORF Clone, Rat, C-GFPSpark® tag RG80442-ACG
    PAI-1 Lentiviral cDNA ORF Clone, Rat, C-GFPSpark® tag RG80442-ACGLN
    PAI-1 cDNA ORF Clone, Rat, C-OFPSpark® tag RG80442-ACR
    PAI-1 cDNA ORF Clone, Rat, C-DDK (Flag®) tag RG80442-CF
    PAI-1 cDNA ORF Clone, Rat, N-DDK (Flag®) tag RG80442-NF
    PAI-1 cDNA ORF Clone, Rat, C-His tag RG80442-CH
    PAI-1 cDNA ORF Clone, Rat, N-His tag RG80442-NH
    PAI-1 cDNA ORF Clone, Rat, C-HA tag RG80442-CY
    PAI-1 cDNA ORF Clone, Rat, C-Myc tag RG80442-CM
    PAI-1 cDNA ORF Clone, Rat, N-Myc tag RG80442-NM
    PAI-1 qPCR Primer Pairs, Rat RP300411

    PAI-1 cDNA ORF Clone, Mouse, Related Products

    Product Name Catalog#(PDF)
    PAI-1 cDNA ORF Clone, Mouse, C-GFPSpark® tag MG57025-ACG
    PAI-1 cDNA ORF Clone, Mouse, N-GFPSpark® tag MG57025-ANG
    PAI-1 cDNA ORF Clone, Mouse, C-Myc tag MG57025-CM

    PAI-1 cDNA ORF Clone, Human, Related Products

    Product Name Catalog#(PDF)
    PAI-1 cDNA ORF Clone in Cloning Vector, Human HG10296-M
    PAI-1 cDNA ORF Clone, Human, untagged HG10296-UT
    PAI-1 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG10296-ACGLN
    PAI-1 cDNA ORF Clone, Human, C-GFPSpark® tag HG10296-ACG
    PAI-1 cDNA ORF Clone, Human, C-OFPSpark® tag HG10296-ACR
    PAI-1 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG10296-CF
    PAI-1 cDNA ORF Clone, Human, N-DDK (Flag®) tag HG10296-NF
    PAI-1 cDNA ORF Clone, Human, C-His tag HG10296-CH
    PAI-1 cDNA ORF Clone, Human, N-His tag HG10296-NH
    PAI-1 cDNA ORF Clone, Human, C-HA tag HG10296-CY
    PAI-1 cDNA ORF Clone, Human, N-HA tag HG10296-NY
    PAI-1 cDNA ORF Clone, Human, C-Myc tag HG10296-CM
    PAI-1 cDNA ORF Clone, Human, N-Myc tag HG10296-NM
    PAI-1 qPCR Primer Pairs, Human HP100341
    info info
    添加购物车成功! 添加购物车失败!