( We provide with SerpinF1 qPCR primers for gene expression analysis, RP300445 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|大鼠 PEDF 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||RG80481-ACG|
|大鼠 PEDF 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||RG80481-ACR|
|大鼠 PEDF 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||RG80481-CF|
|大鼠 PEDF 基因ORF全长cDNA克隆(表达载体), C-His 标签||RG80481-CH|
|大鼠 PEDF 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||RG80481-CM|
|大鼠 PEDF 基因ORF全长cDNA克隆(表达载体), C-HA 标签||RG80481-CY|
|大鼠 PEDF 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||RG80481-NF|
|大鼠 PEDF 基因ORF全长cDNA克隆(表达载体), N-His 标签||RG80481-NH|
|大鼠 PEDF 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||RG80481-NM|
|大鼠 PEDF 基因ORF全长cDNA克隆(表达载体), N-HA 标签||RG80481-NY|
|大鼠 PEDF 基因ORF全长cDNA(克隆载体)||RG80481-U|
|大鼠 PEDF 基因ORF全长cDNA克隆(表达载体)||RG80481-UT|
Pigment epithelium-derived factor, also known as PEDF, Serpin F1, and SERPINF1, is a multiple functional protein which has both anti-angiogenic activity and neurotrophic activity at the same time. PEDF is a secreted glycoprotein that belongs to the noninhibitory serpin. It has an alpha/beta core serine-protease inhibitor domain, three major beta-sheets, and ten alpha-helices. PEDF does not inhibit either serine or cysteine proteinases. PEDF exerts diverse physiological activities including anti-angiogenesis, anti-vasopermeability, anti-tumor, and neurotrophic activities. PEDF acts via multiple high affinity ligands and cell receptors. It has been described as a natural angiogenesis inhibitor with neurotrophic and immune-modulation properties. PEDF induces macrophages apoptosis and necrosis through the activation of peroxisome proliferator-activated receptor-gamma by which PEDF could modulate inflammatory reactions in septic shock. It balances angiogenesis in the eye and blocks tumor progression.