快速订购

大鼠 14-3-3 sigma/Stratifin/YWHAS 基因ORF全长cDNA克隆(表达载体), C-Flag 标签

    产品数据评论相关产品实验方法
    大鼠 SFN cDNA Clone产品信息
    NCBI注册码:XM_001065560.3
    参考序列ORF长度:747bp
    cDNA基因描述:Full length Clone DNA of Rattus norvegicus stratifin with C terminal Flag tag.
    分子别称:Sfn
    分子种属:Rat
    载体:pCMV3-C-FLAG
    质粒:
    限制性酶切位点:
    Tag序列:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
    序列信息:
    测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with SFN qPCR primers for gene expression analysis, RP300518 )
    启动子:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    抗生素(大肠杆菌):Kanamycin
    抗生素(哺乳动物细胞):Hygromycin
    运输方式:Each tube contains lyophilized plasmid.
    储存方法:The lyophilized plasmid can be stored at room temperature for three months.
    FLAG Tag Info

    FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

    A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

    The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

    大鼠 14-3-3 sigma/Stratifin/YWHAS 基因ORF全长cDNA克隆(表达载体), C-Flag 标签 on other vectors
    大鼠 14-3-3 sigma/Stratifin/YWHAS 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签RG80554-ACG1950
    大鼠 14-3-3 sigma/Stratifin/YWHAS 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签RG80554-ACR1950
    大鼠 14-3-3 sigma/Stratifin/YWHAS 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签RG80554-ANG1950
    大鼠 14-3-3 sigma/Stratifin/YWHAS 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签RG80554-ANR1950
    大鼠 14-3-3 sigma/Stratifin/YWHAS 基因ORF全长cDNA克隆(表达载体), C-Flag 标签RG80554-CF1770
    大鼠 14-3-3 sigma/Stratifin/YWHAS 基因ORF全长cDNA克隆(表达载体), C-His 标签RG80554-CH1770
    大鼠 14-3-3 sigma/Stratifin/YWHAS 基因ORF全长cDNA克隆(表达载体), C-Myc 标签RG80554-CM1770
    大鼠 14-3-3 sigma/Stratifin/YWHAS 基因ORF全长cDNA克隆(表达载体), C-HA 标签RG80554-CY1770
    大鼠 14-3-3 sigma/Stratifin/YWHAS 基因ORF全长cDNA克隆(表达载体), N-Flag 标签RG80554-NF1770
    大鼠 14-3-3 sigma/Stratifin/YWHAS 基因ORF全长cDNA克隆(表达载体), N-His 标签RG80554-NH1770
    大鼠 14-3-3 sigma/Stratifin/YWHAS 基因ORF全长cDNA克隆(表达载体), N-Myc 标签RG80554-NM1770
    大鼠 14-3-3 sigma/Stratifin/YWHAS 基因ORF全长cDNA克隆(表达载体), N-HA 标签RG80554-NY1770
    大鼠 14-3-3 sigma/Stratifin/YWHAS 基因ORF全长cDNA(克隆载体)RG80554-U760
    大鼠 14-3-3 sigma/Stratifin/YWHAS 基因ORF全长cDNA克隆(表达载体)RG80554-UT1770
     点击了解更多载体信息
    Product nameProduct name
    研究背景

    14-3-3 protein sigma (YWHAS), also known as stratifin (SFN) and epithelial cell marker protein 1, is a member of the14-3-3 proteins which are a family of conserved regulatory molecules expressed in all eukaryotic cells. The name 14-3-3 refers to the particular elution and migration pattern of these proteins on DEAE-cellulose chromatography and starch-gel electrophoresis. The 14-3-3 proteins eluted in the 14th fraction of bovine brain homogenate and were found on positions 3.3 of subsequent electrophoresis. There are seven genes that encode 14-3-3s in most mammals. 14-3-3 proteins have been identified as adapter proteins implicated in the regulation of a large spectrum of both general and specialized signaling pathway. More than 100 signaling proteins have been reported as 14-3-3 ligands including kinases, phosphatases, and transmembrane receptors, and the binding generally results in the modulation of the activity of the binding partner. YWHAE exists as a homodimer and present mainly in tissues enriched in stratified squamous keratinising epithelium. YWHAS has been repoted to interact with KRT17 and GAB2, and may regulate protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway upon binding to KRT17. Additionally, YWHAS (SFN) may also act as a p53-regulated inhibitor of G2/M progression.

    Size / Price
    货号: RG80554-CF
    目录价: 
    单价:      (You Save: )

    Datasheet & Documentation

    Contact Us
      您曾浏览过的产品
        产品数据可能随批次变化,最新数据请详见随货说明书。
        请注意:所有产品都是“仅用于科研,而不能用于诊断或治疗用途”