|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|大鼠 Osteoprotegerin/TNFRSF11B 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||RG80159-ACG|
|大鼠 Osteoprotegerin/TNFRSF11B 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||RG80159-ACR|
|大鼠 Osteoprotegerin/TNFRSF11B 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||RG80159-CF|
|大鼠 Osteoprotegerin/TNFRSF11B 基因ORF全长cDNA克隆(表达载体), C-His 标签||RG80159-CH|
|大鼠 Osteoprotegerin/TNFRSF11B 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||RG80159-CM|
|大鼠 Osteoprotegerin/TNFRSF11B 基因ORF全长cDNA克隆(表达载体), C-HA 标签||RG80159-CY|
|大鼠 Osteoprotegerin/TNFRSF11B 基因ORF全长cDNA(克隆载体)||RG80159-G|
|大鼠 Osteoprotegerin/TNFRSF11B 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||RG80159-NF|
|大鼠 Osteoprotegerin/TNFRSF11B 基因ORF全长cDNA克隆(表达载体), N-His 标签||RG80159-NH|
|大鼠 Osteoprotegerin/TNFRSF11B 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||RG80159-NM|
|大鼠 Osteoprotegerin/TNFRSF11B 基因ORF全长cDNA克隆(表达载体), N-HA 标签||RG80159-NY|
|大鼠 Osteoprotegerin/TNFRSF11B 基因ORF全长cDNA克隆(表达载体)||RG80159-UT|
Osteoprotegerin or TNFRSF11B is a member of the TNF-receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest that this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined. Osteoprotegerin/TNFRSF11B acts as decoy receptor for RANKL and thereby neutralizes its function in osteoclastogenesis. This protein may inhibit the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local RANKL/OPG ratio. Osteoprotegerin/TNFRSF11B also play a role in preventing arterial calcification, act as decoy receptor for TRAIL and protect against apoptosis. TRAIL binding blocks the inhibition of osteoclastogenesis.