|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|大鼠 GITR/TNFRSF18/CD357 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||RG80192-ACG|
|大鼠 GITR/TNFRSF18/CD357 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||RG80192-ACR|
|大鼠 GITR/TNFRSF18/CD357 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||RG80192-CF|
|大鼠 GITR/TNFRSF18/CD357 基因ORF全长cDNA克隆(表达载体), C-His 标签||RG80192-CH|
|大鼠 GITR/TNFRSF18/CD357 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||RG80192-CM|
|大鼠 GITR/TNFRSF18/CD357 基因ORF全长cDNA克隆(表达载体), C-HA 标签||RG80192-CY|
|大鼠 GITR/TNFRSF18/CD357 基因ORF全长cDNA(克隆载体)||RG80192-G|
|大鼠 GITR/TNFRSF18/CD357 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||RG80192-NF|
|大鼠 GITR/TNFRSF18/CD357 基因ORF全长cDNA克隆(表达载体), N-His 标签||RG80192-NH|
|大鼠 GITR/TNFRSF18/CD357 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||RG80192-NM|
|大鼠 GITR/TNFRSF18/CD357 基因ORF全长cDNA克隆(表达载体), N-HA 标签||RG80192-NY|
|大鼠 GITR/TNFRSF18/CD357 基因ORF全长cDNA克隆(表达载体)||RG80192-UT|
GITR, also known as TNFRSF18(CD357), belongs to the tumor necrosis factor receptor (TNF-R) superfamily. It is the receptor for TNFSF18. GITR plays a key role in dominant immunological self-tolerance maintained by CD25(+)CD4(+) regulatory T cells. GITR may be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. GITR and its ligand are important costimulatory molecules in the pathogenesis of autoimmune diseases. It also mediates NF-kappa-B activation via the TRAF2/NIK pathway.