VRK1 cDNA ORF Clone, Rat, N-DDK (Flag®) tag

Cat: RG81611-NF
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VRK1 cDNA ORF Clone, Rat, N-DDK (Flag®) tag 基本信息
基因
种属
Rat
NCBI 参考序列号
参考序列ORF长度
1245 bp
描述
Full length Clone DNA of Rat vaccinia related kinase 1 with N terminal Flag tag.
质粒
启动子
Enhanced CMV promoter
载体
pCMV3-N-FLAG
标签序列
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

VRK1 Background Information

VRK1 is a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. Serine/threonine protein kinases are tumor suppressor that controls the activity of AMP-activated protein kinase family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. VRK1 contains 1 protein kinase domain and localizes to the nucleus. VRK1 gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. As a serine/threonine kinase, VRK1 phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Defects in VRK1 are the cause of pontocerebellar hypoplasia type 1 (PCH1), also called pontocerebellar hypoplasia with infantile spinal muscular atrophy or pontocerebellar hypoplasia with anterior horn cell disease. PCH1 is characterized by an abnormally small cerebellum and brainstem, central and peripheral motor dysfunction from birth, gliosis and anterior horn cell degeneration resembling infantile spinal muscular atrophy.

Full Name
vaccinia related kinase 1
References
  • Sugimoto J, et al. (1999) Isolation and mapping of a polymorphic CA repeat sequence at the human VRK1 locus. J Hum Genet. 44 (2): 133-4.
  • Lopez-Borges S, et al. (2000) The human vaccinia-related kinase 1 (VRK1) phosphorylates threonine-18 within the mdm-2 binding site of the p53 tumour suppressor protein. Oncogene. 19 (32): 3656-64.
  • Sevilla A, et al. (2004) c-Jun phosphorylation by the human vaccinia-related kinase 1 (VRK1) and its cooperation with the N-terminal kinase of c-Jun (JNK). Oncogene. 23 (55): 8950-8.
  • VRK1 cDNA ORF Clone, Rat, Related Products

    Product Name Catalog#(PDF)
    VRK1 cDNA ORF Clone in Cloning Vector, Rat RG81611-G
    VRK1 cDNA ORF Clone, Rat, untagged RG81611-UT
    VRK1 Lentiviral cDNA ORF Clone, Rat, C-GFPSpark® tag RG81611-ACGLN
    VRK1 cDNA ORF Clone, Rat, C-GFPSpark® tag RG81611-ACG
    VRK1 cDNA ORF Clone, Rat, N-GFPSpark® tag RG81611-ANG
    VRK1 cDNA ORF Clone, Rat, C-OFPSpark® tag RG81611-ACR
    VRK1 cDNA ORF Clone, Rat, N-OFPSpark® tag RG81611-ANR
    VRK1 cDNA ORF Clone, Rat, C-DDK (Flag®) tag RG81611-CF
    VRK1 cDNA ORF Clone, Rat, C-His tag RG81611-CH
    VRK1 cDNA ORF Clone, Rat, N-His tag RG81611-NH
    VRK1 cDNA ORF Clone, Rat, C-HA tag RG81611-CY
    VRK1 cDNA ORF Clone, Rat, N-HA tag RG81611-NY
    VRK1 cDNA ORF Clone, Rat, C-Myc tag RG81611-CM
    VRK1 cDNA ORF Clone, Rat, N-Myc tag RG81611-NM

    VRK1 cDNA ORF Clone, Human, Related Products

    Product Name Catalog#(PDF)
    VRK1 cDNA ORF Clone in Cloning Vector, Human HG11555-M
    VRK1 cDNA ORF Clone, Human, untagged HG11555-UT
    VRK1 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG11555-ACGLN
    VRK1 cDNA ORF Clone, Human, C-GFPSpark® tag HG11555-ACG
    VRK1 cDNA ORF Clone, Human, N-GFPSpark® tag HG11555-ANG
    VRK1 cDNA ORF Clone, Human, C-OFPSpark® tag HG11555-ACR
    VRK1 cDNA ORF Clone, Human, N-OFPSpark® tag HG11555-ANR
    VRK1 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG11555-CF
    VRK1 cDNA ORF Clone, Human, N-DDK (Flag®) tag HG11555-NF
    VRK1 cDNA ORF Clone, Human, C-His tag HG11555-CH
    VRK1 cDNA ORF Clone, Human, N-His tag HG11555-NH
    VRK1 cDNA ORF Clone, Human, C-HA tag HG11555-CY
    VRK1 cDNA ORF Clone, Human, N-HA tag HG11555-NY
    VRK1 cDNA ORF Clone, Human, C-Myc tag HG11555-CM
    VRK1 cDNA ORF Clone, Human, N-Myc tag HG11555-NM
    VRK1 qPCR Primer Pairs, Human HP101661
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