Anti-alpha 1 Antichymotrypsin / SerpinA3 Antibody

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Anti-alpha 1 Antichymotrypsin / SerpinA3 Antibody (Rabbit Polyclonal 抗体) 产品信息

产品名称
Anti-alpha 1 Antichymotrypsin / SerpinA3 Antibody
经验证的应用
WB,ELISA
交叉反应
Reacts with: Human
特异性
Human alpha 1 Antichymotrypsin / SerpinA3
免疫原
Recombinant Human SerpinA3 protein (Catalog#10307-H08H)
制备方法
Produced in rabbits immunized with purified, recombinant Human SerpinA3 (rh SerpinA3; Catalog#10307-H08H; NP_001076.2; Met 1-Ala 423). SerpinA3 specific IgG was purified by human SerpinA3 affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-alpha 1 Antichymotrypsin / SerpinA3 Antibody (Rabbit Polyclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:2000
ELISA 1:1000-1:2000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-alpha 1 Antichymotrypsin / SerpinA3 Antibody (Rabbit Polyclonal 抗体) 图片

Anti-SerpinA3 rabbit polyclonal antibody at 1:500 dilution

Lane A: Hela Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:70 kDa

Observed band size:65 kDa

(We are unsure as to the identity of these extra bands.)

Anti-alpha 1 Antichymotrypsin / SerpinA3 Antibody: 别称

Anti-AACT Antibody; Anti-ACT Antibody; Anti-GIG24 Antibody; Anti-GIG25 Antibody; Anti-MGC88254 Antibody; Anti-SERPINA3 Antibody

alpha 1 Antichymotrypsin / SerpinA3 背景信息

SerpinA3, also known as Alpha 1-antichymotrypsin (AACT), is a plasma alpha globulin glycoprotein, and is a member of serpin superfamily of the serine protease inhibitors consisting of at least 35 members. SerpinA3 has been demonstrated to inhibit the activity of certain serine proteases, such as cathepsin G found in neutrophils, and chymases present in mast cells, by inducing a major conformational rearrangement, and thus protects some tissues from damage caused by proteolytic enzymes. This enzyme is produced primarily in the liver, and is identified as an acute-phase inflammatory protein. SerpinA3 deficiency has been associated with liver disease, and mutations of this gene have been observed in patients with Parkinson disease and chronic obstructive pulmonary disease. In addition, ACT gene polymorphism has been implicated with Alzheimer’s disease (AD), cerebral amyloid angiopathy (CAA), as well as stroke, since SerpinA3 is a major constituent of the plaques in AD and an inhibitor of amyloid beta peptide degradation.
全称
serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3
参考文献
  • Nilsson, L.N. et al., 2001, J. Neurosci. 21: 1444-1451.
  • Ikari, Y. et al., 2001, J. Biol. Chem. 276: 11798-11803.
  • Vila, N. et al., 2000, Stroke. 31: 2103-2105
  • Eriksson, S. et al., 1995, Proc. Natl. Acad. Sci. USA. 92: 2313-2317.
  • Skeel, A. et al., 2001, J. Biol. Chem. 276: 21932-21937.
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