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人 TERF1/TRF1 基因ORF全长cDNA克隆(表达载体)

  • Human TERF1 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged
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 TERF1 cDNA Clone产品信息
NCBI注册码:BC029378
参考序列ORF长度:1260bp
cDNA基因描述:Full length Clone DNA of Homo sapiens telomeric repeat binding factor (NIMA-interacting) 1.
分子别称:TRF, PIN2, TRF1, TRBF1, t-TRF1, hTRF1-AS
分子种属:Human
载体:pCMV/hygro
质粒:pCMV-TERF1
限制性酶切位点:HindIII + XbaI (5.5kb + 1.26kb)
Tag序列:
序列信息:Identical with the Gene Bank Ref. ID sequence.
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
( We provide with TERF1 qPCR primers for gene expression analysis, HP104613 )
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Ampicillin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
 TERF1 基因质粒图
Human TERF1 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged
pCMV/hygro Vector Information
 
Vector Name pCMV/hygro
Vector Size 5657bp
Vector Type Mammalian Expression Vector
Expression Method Constiutive ,Stable / Transient
Promoter CMV
Antibiotic Resistance Ampicillin
Selection In Mammalian Cells Hygromycin
Protein Tag None
Sequencing Primer Forward:T7(TAATACGACTCACTATAGGG)
Reverse:BGH(TAGAAGGCACAGTCGAGG)


Schematic of pCMV/hygro Multiple Cloning Sites
人 TERF1/TRF1 基因ORF全长cDNA克隆(表达载体) on other vectors
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研究背景

Telomeric repeat binding factor 1 (TRF1), also known as TERF1, the shelterin complex, which modulates the telomere structures. TRF1 protein structure contains a C-terminal Myb motif, a dimerization domain near its N-terminus and an acidic N-terminus. Pin2/TRF1 was originally identified as a protein bound to telomeric DNA (TRF1) and as a protein involved in mitotic regulation (Pin2). Pin2/TRF1 negatively regulates telomere length and importantly, its function is tightly regulated during the cell cycle, acting as an important regulator of mitosis. TRF1 can be bound and modulated by two nucleolar GTP-binding proteins, nucleostemin (NS) and guanine nucleotide binding protein-like 3-like (GNL3L), which exhibit apparently opposite effects on the protein degradation of TRF1. TRF1/TERF1 may has association with cancer. TRF1 may play a significant role in cell differentiation in non-small cell lung cancer (NSCLC). The expression level of TRF1 protein is significantly reduced in kidney cancer and the level is negatively correlated with malignant degree of the cancer. TRF1 expression in malignant gliomas cells, may play a role in the malignant progression of astroglial brain tumors.

参考资料
  • Tsai RY. (2009) Nucleolar modulation of TRF1: a dynamic way to regulate telomere and cell cycle by nucleostemin and GNL3L. Cell Cycle. 8(18):2912-6.
  • Chen YC, et al. (2009) Phosphorylation of telomeric repeat binding factor 1 (TRF1) by Akt causes telomere shortening. Cancer Invest. 27(1): 24-8.
  • Hu J, et al. (2006) Expression of telomeric repeat binding factor 1 in non-small cell lung cancer. J Surg Oncol. 93(1): 62-7.
  • La Torre D, et al. (2005) Expression of telomeric repeat binding factor-1 in astroglial brain tumors. Neurosurgery. 56(4): 802-10.
  • Shi JM, et al. (2004) Expression of telomere repeat binding factor 1 (TRF1) protein in kidney cancer. Zhejiang Da Xue Xue Bao Yi Xue Ban. 33(6): 496-9, 508.
  • Zhou XZ, et al. (2003) Role of Pin2/TRF1 in telomere maintenance and cell cycle control. J Cell Biochem. 89(1): 19-37.
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    货号: HG13044-G-N
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